ArticlePloS one2013
QTL analysis using SNP markers developed by next-generation sequencing for identification of candidate genes controlling 4-methylthio-3-butenyl glucosinolate contents in roots of radish, Raphanus sativus L.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- A Comparative Transcriptome and Metabolome Combined Analysis Reveals the Key Genes and Their Regulatory Model Responsible for Glucoraphasatin Accumulation in Radish Fleshy Taproots.International journal of molecular sciences · 2022Article
- Genetic manipulation of anti-nutritional factors in major crops for a sustainable diet in future.Frontiers in plant science · 2022Review
- Sustainable Use of Bioactive Compounds fromMolecules (Basel, Switzerland) · 2021Review
- Article
- Identification of genome-wide single-nucleotide polymorphisms among geographically diverse radish accessions.DNA research : an international journal for rapid publication of reports on genes and genomes · 2020Article
- Fine-mapping of a major QTL (Fwr1) for fusarium wilt resistance in radish.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Differential expression of major genes involved in the biosynthesis of aliphatic glucosinolates in intergeneric Baemoochae (Brassicaceae) and its parents during development.Plant molecular biology · 2020Article
- An ultra-high-density genetic map provides insights into genome synteny, recombination landscape and taproot skin colour in radish (Raphanus sativus L.).Plant biotechnology journal · 2020Article
- Transcriptome analysis for identifying possible gene regulations during maize root emergence and formation at the initial growth stage.Genes & genomics · 2018Article
- High-density genetic mapping of a major QTL for resistance to multiple races of loose smut in a tetraploid wheat cross.PloS one · 2018Article
- A 2-Oxoglutarate-Dependent Dioxygenase Mediates the Biosynthesis of Glucoraphasatin in Radish.Plant physiology · 2017Article
- Review
- Functional analysis of three BrMYB28 transcription factors controlling the biosynthesis of glucosinolates in Brassica rapa.Plant molecular biology · 2016Article
- Quantitative Trait Loci for Morphological Traits and their Association with Functional Genes in Raphanus sativus.Frontiers in plant science · 2016Article
- Isolate Dependency of Brassica rapa Resistance QTLs to Botrytis cinerea.Frontiers in plant science · 2016Article
- Novel glucosinolate composition lacking 4-methylthio-3-butenyl glucosinolate in Japanese white radish (Raphanus sativus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2015Article
- The radish genome and comprehensive gene expression profile of tuberous root formation and development.Scientific reports · 2015Article
- Draft sequences of the radish (Raphanus sativus L.) genome.DNA research : an international journal for rapid publication of reports on genes and genomes · 2014Article
- Glucosinolate metabolism, functionality and breeding for the improvement of Brassicaceae vegetables.Breeding science · 2014Review
- Comparative genomics of Brassicaceae crops.Breeding science · 2014Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SNP markers for QTL analysis of 4-MTB-GSL contents in radish roots were developed by determining nucleotide sequences of bulked PCR products using a next-generation sequencer. DNA fragments were amplified from two radish lines by multiplex PCR with six primer pairs, and those amplified by 2,880 primer pairs were mixed and sequenced. By assembling sequence data, 1,953 SNPs in 750 DNA fragments, 437 of which have been previously mapped in a linkage map, were identified. A linkage map of nine linkage groups was constructed with 188 markers, and five QTLs were detected in two F(2) populations, three of them accounting for more than 50% of the total phenotypic variance being repeatedly detected. In the identified QTL regions, nine SNP markers were newly produced. By synteny analysis of the QTLs regions with Arabidopsis thaliana and Brassica rapa genome sequences, three candidate genes were selected, i.e., RsMAM3 for production of aliphatic glucosinolates linked to GSL-QTL-4, RsIPMDH1 for leucine biosynthesis showing strong co-expression with glucosinolate biosynthesis genes linked to GSL-QTL-2, and RsBCAT4 for branched-chain amino acid aminotransferase linked to GSL-QTL-1. Nucleotide sequences and expression of these genes suggested their possible function in 4MTB-GSL biosynthesis in radish roots.
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