ArticlePloS one2013
Genomic stability of lyophilized sheep somatic cells before and after nuclear transfer.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Stallion Sperm Lyophilisation: Effect of Source of Sperm and Reproductive Season.Veterinary sciences · 2026Article
- Somatic Cell Nuclear Transfer Using Freeze-Dried Protaminized Donor Nuclei.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Healthy cloned offspring derived from freeze-dried somatic cells.Nature communications · 2022Article
- Molecular and Histological Evaluation of Sheep Ovarian Tissue Subjected to Lyophilization.Animals : an open access journal from MDPI · 2021Article
- Whole genome integrity and enhanced developmental potential in ram freeze-dried spermatozoa at mild sub-zero temperature.Scientific reports · 2020Article
- DNA fragmentation in epididymal freeze-dried ram spermatozoa impairs embryo development.The Journal of reproduction and development · 2018Article
- Nuclear quiescence and histone hyper-acetylation jointly improve protamine-mediated nuclear remodeling in sheep fibroblasts.PloS one · 2018Article
- Article
- Production of hand-made cloned buffalo (Bubalus bubalis) embryos from non-viable somatic cells.In vitro cellular & developmental biology. Animal · 2016Article
- Exogenous Expression of Human Protamine 1 (hPrm1) Remodels Fibroblast Nuclei into Spermatid-like Structures.Cell reports · 2015Article
- Different Donor Cell Culture Methods Can Influence the Developmental Ability of Cloned Sheep Embryos.PloS one · 2015Article
- Hope for restoration of dead valuable bulls through cloning using donor somatic cells isolated from cryopreserved semen.PloS one · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
The unprecedented decline of biodiversity worldwide is urging scientists to collect and store biological material from seriously threatened animals, including large mammals. Lyophilization is being explored as a low-cost system for storage in bio-banks of cells that might be used to expand or restore endangered or extinct species through the procedure of Somatic Cell Nuclear Transfer (SCNT). Here we report that the genome is intact in about 60% of lyophylized sheep lymphocytes, whereas DNA damage occurs randomly in the remaining 40%. Remarkably, lyophilized nuclei injected into enucleated oocytes are repaired by a robust DNA repairing activity of the oocytes, and show normal developmental competence. Cloned embryos derived from lyophylized cells exhibited chromosome and cellular composition comparable to those of embryos derived from fresh donor cells. These findings support the feasibility of lyophylization as a storage procedure of mammalian cells to be used for SCNT.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.