ArticleMolecular biology reports2013
Development of a robust, low cost stem-loop real-time quantification PCR technique for miRNA expression analysis.
Article in Molecular biology reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- OM-sqPCR enables one-step multiplex quantification of circulating small-RNA subclasses for colorectal cancer classification.iScience · 2026Article
- In Situ Single-Particle LSPR Biosensor for Amplification-Free Detection of miR-451 via Dark-Field Scattering Spectroscopy.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Computational Discovery of AngiomiRs and Evaluating the Synergistic Effects of Target miRNA with Tranexamic Acid in Colorectal Cancer.Current medicinal chemistry · 2025Article
- Integrated periodontal pathogens and circulating miRNAs: a novel non-invasive panel of biomarkers for pancreatic cancer.Frontiers in cellular and infection microbiology · 2025Article
- An Autocatalytic Platform Combining a Nonlinear Hybridization Chain Reaction and DNAzyme to Detect microRNA.Bio-protocol · 2024Article
- Development of a Modular miRNA-Responsive Biosensor for Organ-Specific Evaluation of Liver Injury.Biosensors · 2024Article
- Upregulation of developmentally-downregulated miR-1247-5p promotes neuroprotection and axon regeneration in vivo.Neuroscience letters · 2024Article
- Integrating rapamycin with novel PI3K/Akt/mTOR inhibitor microRNAs on NOTCH1-driven T-cell acute lymphoblastic leukemia (T-ALL).BioImpacts : BI · 2024Article
- Inhibition of miR-200b-3p confers broad-spectrum resistance to viral infection by targeting TBK1.mBio · 2023Article
- Smartphone-operated affordable PCR thermal cycler for the detection of antimicrobial resistant bacterial genes.PLOS global public health · 2023Article
- Printed Electrochemical Strip for the Detection of miRNA-29a: A Possible Biomarker Related to Alzheimer's Disease.Analytical chemistry · 2022Article
- Studies of Infection and Experimental Reactivation by Recombinant VZV with Mutations in Virally-Encoded Small Non-Coding RNA.Viruses · 2022Article
- The role of miRNA-377 as a tumor suppressor in lung cancer by negative regulation of genes belonging to ErbB signaling pathway.Molecular biology reports · 2022Article
- A Novel Helper qPCR Assay for the Detection of miRNA Using Target/Helper Template for Primer Formation.International journal of analytical chemistry · 2022Article
- Locked-nucleotide antagonists to varicella zoster virus small non-coding RNA block viral growth and have potential as an anti-viral therapy.Antiviral research · 2021Article
- SCMFMDA: Predicting microRNA-disease associations based on similarity constrained matrix factorization.PLoS computational biology · 2021Article
- SNFIMCMDA: Similarity Network Fusion and Inductive Matrix Completion for miRNA-Disease Association Prediction.Frontiers in cell and developmental biology · 2021Article
- ILPMDA: Predicting miRNA-Disease Association Based on Improved Label Propagation.Frontiers in genetics · 2021Article
- Identification of differentially expressed microRNAs in primary esophageal achalasia by next-generation sequencing.Turkish journal of biology = Turk biyoloji dergisi · 2021Article
- MiR-130a-3p blocks Wnt signaling cascade in the triple-negative breast cancer by targeting the key players at multiple points.Heliyon · 2020Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Development of a rapid and accurate quantification method for the detection of microRNAs (miRNAs) has been desired, in particular, when they are differently expressed in normal and pathological conditions. However, various methods for the quantification of small non-coding RNAs as well as miRNAs have been described. These methods mainly include hybridization-based approaches such as primer extension, northern blotting, microarray profiling, and reverse transcription (RT) PCR. Here, we developed a simple and rapid method based on stem-loop primer-based real-time PCR assay for sensitive and accurate detection of mature miRNAs. Initially, a miRNA-specific stem-loop RT primer is used for RT, which is followed by TaqMan real-time PCR assay using specific forward primer in combination with universal reverse primer and TaqMan probe. The assay has shown high sensitivity (≤50 copies/reaction) for miRNA detection in two breast cancer cell lines, MCF-7 and MDA-MB-231. This assay might be implicated as a rapid and cost effective method for the detection of small non-coding RNAs.
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