ArticleMicrobiology (Reading, England)2013
Shuttle vectors for facile gap repair cloning and integration into a neutral locus in Candida albicans.
Article in Microbiology (Reading, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed.
- Article
- Isw2-mediated chromatin remodeling governs antifungal tolerance and heteroresistance in Candida albicans.npj antimicrobials and resistance · 2026Article
- Article
- A CandiChrome toolkit for multicolor labeling ofbioRxiv : the preprint server for biology · 2026Article
- Expansion and optimization of the auxin-inducible degron 2 (AID2) system inbioRxiv : the preprint server for biology · 2026Article
- Response ofmSphere · 2026Article
- Proximity labeling identification of plasma membrane eisosome proteins in Candida albicans.Genetics · 2025Article
- The Cwr1 protein kinase localizes to the plasma membrane and mediates resistance to cell wall stress inmSphere · 2024Article
- Collateral sensitivity counteracts the evolution of antifungal drug resistance in Candida auris.Nature microbiology · 2024Article
- Candida albicans pathways that protect against organic peroxides and lipid peroxidation.PLoS genetics · 2024Article
- A fungal metabolic regulator underlies infectious synergism during Candida albicans-Staphylococcus aureus intra-abdominal co-infection.Nature communications · 2024Article
- Sur7 mediates a novel pathway for PIMolecular biology of the cell · 2024Article
- Article
- Article
- ThemSphere · 2023Article
- Rapid, efficient auxin-inducible protein degradation inmSphere · 2023Article
- Rewilding of laboratory mice enhances granulopoiesis and immunity through intestinal fungal colonization.Science immunology · 2023Article
- Rapid, efficient auxin-inducible protein degradation inbioRxiv : the preprint server for biology · 2023Article
- Article
- Development and applications of a CRISPR activation system for facile genetic overexpression in Candida albicans.G3 (Bethesda, Md.) · 2023Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
Abstract
Candida albicans is the most prevalent fungal pathogen of humans. The current techniques used to construct C. albicans strains require integration of exogenous DNA at ectopic locations, which can exert position effects on gene expression that can confound the interpretation of data from critical experiments such as virulence assays. We have identified a large intergenic region, NEUT5L, which facilitates the integration and expression of ectopic genes. To construct and integrate inserts into this novel locus, we re-engineered yeast/bacterial shuttle vectors by incorporating 550 bp of homology to NEUT5L. These vectors allow rapid, facile cloning through in vivo recombination (gap repair) in Saccharomyces cerevisiae and efficient integration of the construct into the NEUT5L locus. Other useful features of these vectors include a choice of three selectable markers (URA3, the recyclable URA3-dpl200 or NAT1), and rare restriction enzyme recognition sites for releasing the insert from the vector prior to transformation into C. albicans, thereby reducing the insert size and preventing integration of non-C. albicans DNA. Importantly, unlike the commonly used RPS1/RP10 locus, integration at NEUT5L has no negative effect on growth rates and allows native-locus expression levels, making it an ideal genomic locus for the integration of exogenous DNA in C. albicans.
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