ArticlePlant cell reports2013
An E8 promoter-HSP terminator cassette promotes the high-level accumulation of recombinant protein predominantly in transgenic tomato fruits: a case study of miraculin.
Article in Plant cell reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Review
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- Arabidopsis as a model for translational research.The Plant cell · 2025Review
- Review of methods for detection and characterization of non-tuberculous mycobacteria in aquatic organisms.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Review
- Plant terminators: the unsung heroes of gene expression.Journal of experimental botany · 2023Review
- Plant Promoters and Terminators for High-Precision Bioengineering.Biodesign research · 2023Review
- Different Fruit-Specific Promoters DriveMolecules (Basel, Switzerland) · 2022Article
- Identification of a Transferrable Terminator Element That Inhibits Small RNA Production and Improves Transgene Expression Levels.Frontiers in plant science · 2022Article
- The accumulation of recombinant miraculin is independent of fruit size in tomato.Plant biotechnology (Tokyo, Japan) · 2021Article
- Challenges and Prospects of New Plant Breeding Techniques for GABA Improvement in Crops: Tomato as an Example.Frontiers in plant science · 2020Review
- Plant 3' Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression.Frontiers in plant science · 2020Review
- Production of Marker-Free Apple Plants Expressing the Supersweet Protein Gene Driven by Plant Promoter.Frontiers in plant science · 2019Article
- Transcriptional enhancement of a bacterialPlant biotechnology (Tokyo, Japan) · 2018Article
- Fruit preferential activity of the tomato RIP1 gene promoter in transgenic tomato and Arabidopsis.Molecular genetics and genomics : MGG · 2017Article
- Activating glutamate decarboxylase activity by removing the autoinhibitory domain leads to hyper γ-aminobutyric acid (GABA) accumulation in tomato fruit.Plant cell reports · 2017Article
- The pineapple AcMADS1 promoter confers high level expression in tomato and Arabidopsis flowering and fruiting tissues, but AcMADS1 does not complement the tomato LeMADS-RIN (rin) mutant.Plant molecular biology · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageThe E8 promoter-HSP terminator expression cassette is a powerful tool for increasing the accumulation of recombinant protein in a ripening tomato fruit. Strong, tissue-specific transgene expression is a desirable feature in transgenic plants to allow the production of variable recombinant proteins. The expression vector is a key tool to control the expression level and site of transgene and recombinant protein expression in transgenic plants. The combination of the E8 promoter, a fruit-ripening specific promoter, and a heat shock protein (HSP) terminator, derived from heat shock protein 18.2 of Arabidopsis thaliana, produces the strong and fruit-specific accumulation of recombinant miraculin in transgenic tomato. Miraculin gene expression was driven by an E8 promoter and HSP terminator cassette (E8-MIR-HSP) in transgenic tomato plants, and the miraculin concentration was the highest in the ripening fruits, representing 30-630 μg miraculin of the gram fresh weight. The highest level of miraculin concentration among the transgenic tomato plant lines containing the E8-MIR-HSP cassette was approximately four times higher than those observed in a previous study using a constitutive 35S promoter and NOS terminator cassette (Hiwasa-Tanase et al. in Plant Cell Rep 30:113-124, 2011). These results demonstrate that the combination of the E8 promoter and HSP terminator cassette is a useful tool to increase markedly the accumulation of recombinant proteins in a ripening fruit-specific manner.
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