ArticleNature protocols2013
Derivation of human embryonic stem cells using a post-inner cell mass intermediate.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Extended In Vitro Culture of Human Embryos.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Primate amnion development.Development (Cambridge, England) · 2024Review
- Tissue of Origin, but Not XCI State, Influences Germ Cell Differentiation from Human Pluripotent Stem Cells.Cells · 2021Article
- Review
- Spatially resolved cell polarity proteomics of a human epiblast model.Science advances · 2021Article
- Human blastocyst outgrowths recapitulate primordial germ cell specification events.Molecular human reproduction · 2019Article
- Opening the black box: Stem cell-based modeling of human post-implantation development.The Journal of cell biology · 2019Review
- Metabolic plasticity complements the unique nature and demands of distinct pluripotency states.Stem cell investigation · 2017Article
- Non-invasive methods for embryo selection.Facts, views & vision in ObGyn · 2016Review
- Application Of Small Molecules Favoring Naïve Pluripotency during Human Embryonic Stem Cell Derivation.Cellular reprogramming · 2015Article
- Stem cell therapy in spinal cord injury: Hollow promise or promising science?Journal of craniovertebral junction & spineArticle
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Little is known about the true developmental origin of human embryonic stem cells (hESCs) or the events that initiate their generation. Recently, we have shown that hESCs originate from a post-inner cell mass (ICM) intermediate (PICMI), a unique transient epiblast-like structure that is different from both its ICM progenitor and its subsequent hESC fate. As a closer progenitor of hESCs than the ICM, the PICMI could be used to provide further insight into the human pluripotent state. Here we provide a detailed (7-d) protocol for the culture of the human preimplantation embryos in order to derive the PICMI. Subsequent identification and cryopreservation of the PICMI are described, in addition to hESC derivation. The initial hESC outgrowth is visible within 2-7 d after PICMI plating. By using the protocol provided, we observed PICMI formation in 21.3% of plated blastocysts with good-quality ICMs. Of the PICMIs used for hESC derivation, 80.6% showed hESC outgrowth after further culture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.