ArticleParasitology research2013
Real-time PCR method for the detection and quantification of Acanthamoeba species in various types of water samples.
Article in Parasitology research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Prevalence of free-living amoebae in swimming pools and recreational waters, a systematic review and meta-analysis.Parasitology research · 2022Pooled it
- Genotyping and Molecular Identification of Acanthamoeba Genotype T4 and Naegleria fowleri from Cerebrospinal Fluid Samples of Patients in Turkey: Is it the Pathogens of Unknown Causes of Death?Acta parasitologica · 2022Article
- Efficient nested-PCR-based method development for detection and genotype identification of Acanthamoeba from a small volume of aquatic environmental sample.Scientific reports · 2021Article
- Article
- The role of domestic tap water on Acanthamoeba keratitis in non-contact lens wearers and validation of laboratory methods.Parasitology research · 2015Article
- Development and application of an eDNA method to detect and quantify a pathogenic parasite in aquatic ecosystems.Ecological applications : a publication of the Ecological Society of America · 2015Article
- Seasonal distribution of potentially pathogenic Acanthamoeba species from drinking water reservoirs in Taiwan.Environmental science and pollution research international · 2015Article
- An update on Acanthamoeba keratitis: diagnosis, pathogenesis and treatment.Parasite (Paris, France) · 2015Review
- Density of environmental Acanthamoeba and their responses to superheating disinfection.Parasitology research · 2013Article
- Diversity and seasonal impact of Acanthamoeba species in a subtropical rivershed.BioMed research international · 2013Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, a quantitative real-time PCR was developed to detect and quantify Acanthamoeba spp. in various environmental water samples. The water samples were taken from watershed, water treatment plant, and three thermal spring recreation areas. The overall detection rate was 14.2 % (25/176) for Acanthamoeba spp. The percentages of samples containing Acanthamoeba spp. from river water, raw drinking water, and thermal spring water were 13 % (13/100), 25 % (7/28), and 10.4 % (5/48), respectively. Acanthamoeba spp. concentrations were determined according to SYBR Green quantitative real-time PCR. A plasmid-based standard curve was constructed to determine the Acanthamoeba concentration using dilution factors for achieving 1.36 × 10(9) gene copies per PCR for 18S rRNA gene in Acanthamoeba spp. The resulting concentrations varied by the type of water, in the range of 46-2.6 × 10(2) cells/l in positive raw drinking water, 2.7 × 10(2)-1.5 × 10(4) cells/l in river water, and 54-1.7 × 10(3) cells/l in thermal spring water. The presence of Acanthamoeba spp. in the raw drinking water samples was also found to have a significant difference with heterotrophic plate count. The presence of Acanthamoeba spp. in various aquatic environments may be a potential health hazard and must be further evaluated.
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