ArticleThe Journal of biological chemistry2013
Molecular disruption of the power stroke in the ATP-binding cassette transport protein MsbA.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Native mass spectrometry and structural studies reveal modulation of MsbA-nucleotide interactions by lipids.Nature communications · 2024Article
- Probing the allosteric NBD-TMD crosstalk in the ABC transporter MsbA by solid-state NMR.Communications biology · 2024Article
- The Alternating Access Mechanism in Mammalian Multidrug Resistance Transporters and Their Bacterial Homologs.Membranes · 2023Article
- Biosensor for Multimodal Characterization of an Essential ABC Transporter for Next-Generation Antibiotic Research.ACS applied materials & interfaces · 2023Article
- Breaking down the cell wall: Still an attractive antibacterial strategy.Frontiers in microbiology · 2022Review
- Energetics of lipid transport by the ABC transporter MsbA is lipid dependent.Communications biology · 2021Article
- The molecular evolution of function in the CFTR chloride channel.The Journal of general physiology · 2021Article
- Optimization of Membrane Protein TmrA Purification Procedure Guided by Analytical Ultracentrifugation.Membranes · 2021Article
- A Conserved Motif in Intracellular Loop 1 Stabilizes the Outward-Facing Conformation of TmrAB.Journal of molecular biology · 2021Article
- Complexities of a protonatable substrate in measurements of Hoechst 33342 transport by multidrug transporter LmrP.Scientific reports · 2020Article
- G551D mutation impairs PKA-dependent activation of CFTR channel that can be restored by novel GOF mutations.American journal of physiology. Lung cellular and molecular physiology · 2020Article
- Defining the mechanism of the mitochondrial Atm1p [2Fe-2S] cluster exporter.Metallomics : integrated biometal science · 2020Article
- The extracellular gate shapes the energy profile of an ABC exporter.Nature communications · 2019Article
- Flexible-to-rigid transition is central for substrate transport in the ABC transporter BmrA fromCommunications biology · 2019Article
- Disrupting Gram-Negative Bacterial Outer Membrane Biosynthesis through Inhibition of the Lipopolysaccharide Transporter MsbA.Antimicrobial agents and chemotherapy · 2018Article
- Comparison of mechanistic transport cycle models of ABC exporters.Biochimica et biophysica acta. Biomembranes · 2018Review
- Review
- Article
- Unidirectional Transport Mechanism in an ATP Dependent Exporter.ACS central science · 2017Article
- Specific stabilization of CFTR by phosphatidylserine.Biochimica et biophysica acta. Biomembranes · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
ATP-binding cassette transporters affect drug pharmacokinetics and are associated with inherited human diseases and impaired chemotherapeutic treatment of cancers and microbial infections. Current alternating access models for ATP-binding cassette exporter activity suggest that ATP binding at the two cytosolic nucleotide-binding domains provides a power stroke for the conformational switch of the two membrane domains from the inward-facing conformation to the outward-facing conformation. In outward-facing crystal structures of the bacterial homodimeric ATP-binding cassette transporters MsbA from gram-negative bacteria and Sav1866 from Staphylococcus aureus, two transmembrane helices (3 and 4) in the membrane domains have their cytoplasmic extensions in close proximity, forming a tetrahelix bundle interface. In biochemical experiments on MsbA from Escherichia coli, we show for the first time that a robust network of inter-monomer interactions in the tetrahelix bundle is crucial for the transmission of nucleotide-dependent conformational changes to the extracellular side of the membrane domains. Our observations are the first to suggest that modulation of tetrahelix bundle interactions in ATP-binding cassette exporters might offer a potent strategy to alter their transport activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.