ArticleThe Journal of biological chemistry2013
RegB kinase activity is repressed by oxidative formation of cysteine sulfenic acid.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed.
- Persulfidation of Human CystathionineAntioxidants (Basel, Switzerland) · 2024Article
- XRE transcription factors conserved in Caulobacter and φCbK modulate adhesin development and phage production.PLoS genetics · 2023Article
- Redox Brake Regulator RedB and FnrL Function as Yin-Yang Regulators of Anaerobic-Aerobic Metabolism in Rhodobacter capsulatus.Microbiology spectrum · 2022Article
- A cryptic transcription factor regulates Caulobacter adhesin development.PLoS genetics · 2022Article
- PioABC-Dependent Fe(II) Oxidation during Photoheterotrophic Growth on an Oxidized Carbon Substrate Increases Growth Yield.Applied and environmental microbiology · 2022Article
- The Response Regulator RegA Is a Copper Binding Protein That Covalently Dimerizes When Exposed to Oxygen.Microorganisms · 2022Article
- HAntioxidants (Basel, Switzerland) · 2021Article
- Endogenous SORedox biology · 2021Article
- RegAB Homolog of Burkholderia pseudomallei is the Master Regulator of Redox Control and involved in Virulence.PLoS pathogens · 2021Article
- Adaptation of Dinoroseobacter shibae to oxidative stress and the specific role of RirA.PloS one · 2021Article
- The SrrAB two-component system regulatesProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Regulation of bacterial surface attachment by a network of sensory transduction proteins.PLoS genetics · 2019Article
- Article
- Triphenylphosphonium-Derived Protein Sulfenic Acid Trapping Agents: Synthesis, Reactivity, and Effect on Mitochondrial Function.Chemical research in toxicology · 2019Article
- Characterization of a Glycyl Radical Enzyme Bacterial Microcompartment Pathway inJournal of bacteriology · 2019Article
- Transcriptomic analysis of aerobic respiratory and anaerobic photosynthetic states inMicrobial genomics · 2017Article
- The Global Redox Responding RegB/RegA Signal Transduction System Regulates the Genes Involved in Ferrous Iron and Inorganic Sulfur Compound Oxidation of the AcidophilicFrontiers in microbiology · 2017Article
- Atypical modes of bacterial histidine kinase signaling.Molecular microbiology · 2017Review
- The RegA regulon exhibits variability in response to altered growth conditions and differs markedly betweenMicrobial genomics · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
RegB/RegA comprise a global redox-sensing signal transduction system utilized by a wide range of proteobacteria to sense environmental changes in oxygen tension. The conserved cysteine 265 in the sensor kinase RegB was previously reported to form an intermolecular disulfide bond under oxidizing conditions that converts RegB from an active dimer into an inactive tetramer. In this study, we demonstrate that a stable sulfenic acid (-SOH) derivative also forms at Cys-265 in vitro and in vivo when RegB is exposed to oxygen. This sulfenic acid modification is reversible and stable in the air. Autophosphorylation assay shows that reduction of the SOH at Cys-265 to a free thiol (SH) can increase RegB kinase activity in vitro. Our results suggest that a sulfenic acid modification at Cys-265 performs a regulatory role in vivo and that it may be the major oxidation state of Cys-265 under aerobic conditions. Cys-265 thus functions as a complex redox switch that can form multiple thiol modifications in response to different redox signals to control the kinase activity of RegB.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.