ReviewInternational journal of molecular sciences2013
From protein engineering to immobilization: promising strategies for the upgrade of industrial enzymes.
Review in International journal of molecular sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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Who cites it
81 citing papers in PubMed, 442 citations in OpenAlex.
- Isolation of a lipase-producing strain from yuncheng salt lake and enhancement of lipase activity via UV mutagenesis.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Synthesis and characterization of ZrFeNanoscale advances · 2026Article
- Cadaverine modulates bovine alkaline phosphatase kinetics and structure: spectroscopic and bioinformatics study.Scientific reports · 2026Article
- Prospects and problems in enzyme immobilization methodology: comprehensive review.Biophysical reviews · 2025Review
- Characterization of β-glucosidase activity of a Lactiplantibacillus plantarum 6-phospho-β-glucosidase.Applied microbiology and biotechnology · 2025Article
- Review
- Microbial lipases: advances in production, purification, biochemical characterization, and multifaceted applications in industry and medicine.Microbial cell factories · 2025Review
- Utilization of Fusarium Solani lipase for enrichment of polyunsaturated Omega-3 fatty acids.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Immobilized Cellulase on NHApplied biochemistry and biotechnology · 2024Article
- Autochthonous psychrophilic hydrocarbonoclastic bacteria and its ecological function in contaminated cold environments.Biodegradation · 2024Review
- Enzymes Immobilized into Starch- and Gelatin-Based Hydrogels: Properties and Application in Inhibition Assay.Micromachines · 2023Review
- Facile Purification and Use of Tobamoviral Nanocarriers for Antibody-Mediated Display of a Two-Enzyme System.Viruses · 2023Article
- Curcumin-Sodium Alginate and Curcumin-Chitosan Conjugates as Drug Delivery Systems: An Interesting Rheological Behaviour.Molecules (Basel, Switzerland) · 2023Article
- Enhanced Inhibition of Amyloid Formation by Heat Shock Protein 90 Immobilized on Nanoparticles.ACS chemical neuroscience · 2023Article
- Direct Biocatalytic Processes for COMolecules (Basel, Switzerland) · 2023Review
- An Accessible Method to Improve the Stability and Reusability of Porcine Pancreatic α-Amylase via Immobilization in Gellan-Based Hydrogel Particles Obtained by Ionic Cross-Linking with MgMolecules (Basel, Switzerland) · 2023Article
- Preparation and quality evaluation of sardines sauce rich in omega-3 using the combination of pineapple fruit extracts and fermentation times.Journal of food science and technology · 2023Article
- Tyrosinase Immobilization Strategies for the Development of Electrochemical Biosensors-A Review.Nanomaterials (Basel, Switzerland) · 2023Review
- Multienzyme Coimmobilization on Triheterofunctional Supports.Biomacromolecules · 2023Article
- Target-template relationships in protein structure prediction and their effect on the accuracy of thermostability calculations.Protein science : a publication of the Protein Society · 2023Article
21 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enzymes found in nature have been exploited in industry due to their inherent catalytic properties in complex chemical processes under mild experimental and environmental conditions. The desired industrial goal is often difficult to achieve using the native form of the enzyme. Recent developments in protein engineering have revolutionized the development of commercially available enzymes into better industrial catalysts. Protein engineering aims at modifying the sequence of a protein, and hence its structure, to create enzymes with improved functional properties such as stability, specific activity, inhibition by reaction products, and selectivity towards non-natural substrates. Soluble enzymes are often immobilized onto solid insoluble supports to be reused in continuous processes and to facilitate the economical recovery of the enzyme after the reaction without any significant loss to its biochemical properties. Immobilization confers considerable stability towards temperature variations and organic solvents. Multipoint and multisubunit covalent attachments of enzymes on appropriately functionalized supports via linkers provide rigidity to the immobilized enzyme structure, ultimately resulting in improved enzyme stability. Protein engineering and immobilization techniques are sequential and compatible approaches for the improvement of enzyme properties. The present review highlights and summarizes various studies that have aimed to improve the biochemical properties of industrially significant enzymes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.