ArticleBMJ open2013
A pilot study of rapid whole-genome sequencing for the investigation of a Legionella outbreak.
Article in BMJ open, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
What it found
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Who cites it
68 citing papers in PubMed, 114 citations in OpenAlex.
- Article
- Article
- Phylogenetic Analysis of an HIV Outbreak in a Dialysis Unit at a Tertiary Care Hospital in Multan, Pakistan.Viruses · 2026Article
- Role of Next-Generation Sequencing in Excluding the Nosocomial Origin of a Case of Legionnaires' Disease Integrating Environmental Surveillance and Clinical Diagnosis.Microorganisms · 2026Article
- Leveraging illumina iSeq100 for whole genome sequencing of Salmonella Typhi for a resource-limited setting.Scientific reports · 2025Article
- Comparative genome analysis investigation of nosocomial and community-acquired cases of Legionnaires' disease caused by ST2858 and ST378.Microbiology spectrum · 2025Article
- Comparison of whole genome sequencing typing tools for the typing of Belgian Legionella pneumophila outbreaks isolates.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025Article
- Evaluating Fourier-transform infrared spectroscopy with IR Biotyper as a faster and simpler method for investigating the sources of an outbreak of legionellosis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- High performanceApplied and environmental microbiology · 2024Article
- Long-read sequencing reveals genomic diversity and associated plasmid movement of carbapenemase-producing bacteria in a UK hospital over 6 years.Microbial genomics · 2023Article
- Rapid adaptations ofMicrobial genomics · 2023Article
- Comparative Genomics ofMicroorganisms · 2023Article
- Whole-genome sequencing of the clinical isolate ofEuropean clinical respiratory journal · 2023Article
- Culture-Free Phylogenetic Analysis of Legionella pneumophila Using Targeted CRISPR/Cas9 Next-Generation Sequencing.Microbiology spectrum · 2022Article
- Development of Single Nucleotide Polymorphism (SNP)-Based Triplex PCR Marker for Serotype-specificPathogens (Basel, Switzerland) · 2022Article
- Non-IDCases · 2022Article
- Genomic characterization and assessment of pathogenic potential ofFrontiers in microbiology · 2022Article
- Characterization ofPathogens (Basel, Switzerland) · 2020Article
- Integrating multiple genomic technologies to investigate an outbreak of carbapenemase-producing Enterobacter hormaechei.Nature communications · 2020Article
- Recent trends in molecular diagnostics of yeast infections: from PCR to NGS.FEMS microbiology reviews · 2019Review
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
objectivesEpidemiological investigations of Legionnaires' disease outbreaks rely on the rapid identification and typing of clinical and environmental Legionella isolates in order to identify and control the source of infection. Rapid bacterial whole-genome sequencing (WGS) is an emerging technology that has the potential to rapidly discriminate outbreak from non-outbreak isolates in a clinically relevant time frame.
methodsWe performed a pilot study to determine the feasibility of using bacterial WGS to differentiate outbreak from non-outbreak isolates collected during an outbreak of Legionnaires' disease. Seven Legionella isolates (three clinical and four environmental) were obtained from the reference laboratory and sequenced using the Illumina MiSeq platform at Addenbrooke's Hospital, Cambridge. Bioinformatic analysis was performed blinded to the epidemiological data at the Wellcome Trust Sanger Institute.
resultsWe were able to distinguish outbreak from non-outbreak isolates using bacterial WGS, and to confirm the probable environmental source. Our analysis also highlighted constraints, which were the small number of Legionella pneumophila isolates available for sequencing, and the limited number of published genomes for comparison.
conclusionsWe have demonstrated the feasibility of using rapid WGS to investigate an outbreak of Legionnaires' disease. Future work includes building larger genomic databases of L pneumophila from both clinical and environmental sources, developing automated data interpretation software, and conducting a cost-benefit analysis of WGS versus current typing methods.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.