ArticleBiochemistry2013
Ensemble analysis of primary microRNA structure reveals an extensive capacity to deform near the Drosha cleavage site.
Article in Biochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed.
- Genomic variation of human microRNAs and its association with functional features.Cellular and molecular life sciences : CMLS · 2025Article
- Elucidating the Role of Microprocessor Protein DGCR8 in Bending RNA Structures.Biophysical journal · 2020Article
- Cryo-EM Structures of Human Drosha and DGCR8 in Complex with Primary MicroRNA.Molecular cell · 2020Article
- The roles of structural dynamics in the cellular functions of RNAs.Nature reviews. Molecular cell biology · 2019Review
- Sequence-selective recognition of double-stranded RNA and enhanced cellular uptake of cationic nucleobase and backbone-modified peptide nucleic acids.RNA (New York, N.Y.) · 2017Article
- Article
- Structural dynamics control the MicroRNA maturation pathway.Nucleic acids research · 2016Article
- Inhibition of MiR-92a May Protect Endothelial Cells After Acute Myocardial Infarction in Rats: Role of KLF2/4.Medical science monitor : international medical journal of experimental and clinical research · 2016Article
- Design of Effective Primary MicroRNA Mimics With Different Basal Stem Conformations.Molecular therapy. Nucleic acids · 2016Article
- Article
- Structure based approaches for targeting non-coding RNAs with small molecules.Current opinion in structural biology · 2015Review
- Article
- A central role for the primary microRNA stem in guiding the position and efficiency of Drosha processing of a viral pri-miRNA.RNA (New York, N.Y.) · 2014Article
- Ribonuclease III mechanisms of double-stranded RNA cleavage.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Most noncoding RNAs function properly only when folded into complex three-dimensional (3D) structures, but the experimental determination of these structures remains challenging. Understanding of primary microRNA (miRNA) maturation is currently limited by a lack of determined structures for nonprocessed forms of the RNA. SHAPE chemistry efficiently determines RNA secondary structural information with single-nucleotide resolution, providing constraints suitable for input into MC-Pipeline for refinement of 3D structure models. Here we combine these approaches to analyze three structurally diverse primary microRNAs, revealing deviations from canonical double-stranded RNA structure in the stem adjacent to the Drosha cut site for all three. The necessity of these deformable sites for efficient processing is demonstrated through Drosha processing assays. The structure models generated herein support the hypothesis that deformable sequences spaced roughly once per turn of A-form helix, created by noncanonical structure elements, combine with the necessary single-stranded RNA-double-stranded RNA junction to define the correct Drosha cleavage site.
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Registered trials
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