ArticleFluids and barriers of the CNS2013
Culture models to study leukocyte trafficking across the choroid plexus.
Article in Fluids and barriers of the CNS, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Hyphal penetration is the major pathway of translocation of Candida albicans across the blood-cerebrospinal fluid barrier.Fluids and barriers of the CNS · 2025Article
- In vitro models of the choroid plexus and the blood-cerebrospinal fluid barrier: advances, applications, and perspectives.Human cell · 2024Review
- IL-8 (CXCL8) Correlations with Psychoneuroimmunological Processes and Neuropsychiatric Conditions.Journal of personalized medicine · 2024Review
- Oncostatin M triggers brain inflammation by compromising blood-brain barrier integrity.Acta neuropathologica · 2022Article
- Immortalized human choroid plexus endothelial cells enable an advanced endothelial-epithelial two-cell typeiScience · 2022Article
- Glymphatic System in the Central Nervous System, a Novel Therapeutic Direction Against Brain Edema After Stroke.Frontiers in aging neuroscience · 2021Article
- Localization of ZIP14 and ZIP8 in HIBCPP Cells.Brain sciences · 2020Article
- Method for Primary Epithelial Cell Culture from the Rat Choroid Plexus.Bio-protocol · 2020Article
- Human CD4Fluids and barriers of the CNS · 2020Article
- The Choroid Plexus Is Permissive for a Preactivated Antigen-Experienced Memory B-Cell Subset in Multiple Sclerosis.Frontiers in immunology · 2020Article
- Distinct migratory pattern of naive and effector T cells through the blood-CSF barrier following Echovirus 30 infection.Journal of neuroinflammation · 2019Article
- Optimized cultivation of porcine choroid plexus epithelial cells, a blood-cerebrospinal fluid barrier model, for studying granulocyte transmigration.Laboratory investigation; a journal of technical methods and pathology · 2019Article
- Neuroinvasion and Inflammation in Viral Central Nervous System Infections.Mediators of inflammation · 2016Review
- Cross-reactivity of Antibodies Directed to the Gram-Negative Bacterium Neisseria gonorrhoeae With Heat Shock Protein 60 and ATP-Binding Protein Correlates to Reduced Mitochondrial Activity in HIBCPP Choroid Plexus Papilloma Cells.Journal of molecular neuroscience : MN · 2015Article
- The choroid plexus-a multi-role player during infectious diseases of the CNS.Frontiers in cellular neuroscience · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelled
backgroundA critical point during the course of central nervous system infection is the influx of leukocytes from the blood into the brain across the blood-brain barrier (BBB) and the blood-cerebrospinal fluid barrier (BCSFB). However, experimental in vitro models to investigate leukocyte transmigration across cultured choroid plexus epithelial cells have been lacking so far.
methodsWe have developed a porcine and human "inverted" culture insert system that enables leukocyte transmigration specifically from the physiologically relevant basolateral side. The models use primary porcine choroid plexus epithelial cells (PCPEC) and human choroid plexus papilloma cells (HIBCPP). As a prerequisite for a functional barrier, we optimized culture conditions in which cells are maintained in serum-containing medium until high barrier function is reached. Leukocyte transmigration through the plexus epithelial cells is analysed by three-dimensional Apotome®-imaging and electron microscopy, and the route of transmigration through the plexus epithelial cells, i.e. transcellular as well as paracellular, can be determined. DISCUSSION: As a functionally relevant porcine and human BCSFB model, PCPEC and HIBCPP respectively, offer a wide range of options for analysis of disease-related mechanisms at the choroid plexus epithelium, especially involving human pathogens. Moreover, our in vitro models facilitate the investigation of leukocyte entry into the CNS via the blood-CSF barrier.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.