ArticleNucleic acids research2013
Transcription-independent role for human mitochondrial RNA polymerase in mitochondrial ribosome biogenesis.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 40 citations in OpenAlex.
- POLRMT overexpression increases mtDNA transcription without affecting steady-state mRNA levels.Life science alliance · 2025Article
- The role of m5C, m1A and m7G modifications in tumors of urinary system.Frontiers in cell and developmental biology · 2025Review
- Assessing the Role of Post-Translational Modifications of Mitochondrial RNA Polymerase.International journal of molecular sciences · 2023Article
- Let's make it clear: systematic exploration of mitochondrial DNA- and RNA-protein complexes by complexome profiling.Nucleic acids research · 2023Article
- Quality Matters? The Involvement of Mitochondrial Quality Control in Cardiovascular Disease.Frontiers in cell and developmental biology · 2021Review
- Review
- The DEAD-box helicase Mss116 plays distinct roles in mitochondrial ribogenesis and mRNA-specific translation.Nucleic acids research · 2017Article
- Mitochondrial protein interactome elucidated by chemical cross-linking mass spectrometry.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Methylation associated transcriptional repression of ELOVL5 in novel colorectal cancer cell lines.PloS one · 2017Article
- Mitochondrial transcription in mammalian cells.Frontiers in bioscience (Landmark edition) · 2017Review
- Mitochondrial BMI1 maintains bioenergetic homeostasis in cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016Article
- Multi-focal control of mitochondrial gene expression by oncogenic MYC provides potential therapeutic targets in cancer.Oncotarget · 2016Article
- Mitochondrial pathways to cardiac recovery: TFAM.Heart failure reviews · 2016Review
- Mitochondrial Ribosomal Protein L12 Is Required for POLRMT Stability and Exists as Two Forms Generated by Alternative Proteolysis during Import.The Journal of biological chemistry · 2016Article
- Auditory Pathology in a Transgenic mtTFB1 Mouse Model of Mitochondrial Deafness.The American journal of pathology · 2015Article
- Targeting mitochondrial RNA polymerase in acute myeloid leukemia.Oncotarget · 2015Article
- Transcriptome analysis of complex I-deficient patients reveals distinct expression programs for subunits and assembly factors of the oxidative phosphorylation system.BMC genomics · 2015Article
- Mitochondrial Transcription Factor A (TFAM) Binds to RNA Containing 4-Way Junctions and Mitochondrial tRNA.PloS one · 2015Article
- Mitochondrial protein synthesis: figuring the fundamentals, complexities and complications, of mammalian mitochondrial translation.FEBS letters · 2014Review
- The DEAD box protein Mrh4 functions in the assembly of the mitochondrial large ribosomal subunit.Cell metabolism · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Human mitochondrial RNA polymerase, POLRMT, is required for mitochondrial DNA (mtDNA) transcription and forms initiation complexes with human mitochondrial transcription factor B2 (h-mtTFB2). However, POLRMT also interacts with the paralogue of h-mtTFB2, h-mtTFB1, which is a 12S ribosomal RNA methyltransferase required for small (28S) mitochondrial ribosome subunit assembly. Herein, we show that POLRMT associates with h-mtTFB1 in 28S mitochondrial ribosome complexes that are stable in the absence of mitochondrial transcription and distinct from transcription complexes containing POLRMT and h-mtTFB2. Overexpression of POLRMT in HeLa cells increases 12S rRNA methylation by h-mtTFB1 and reduces the steady-state levels of mtDNA-encoded proteins and respiration, apparently because of a decrease in fully assembled 55S mitochondrial ribosomes. We propose that POLRMT interacts directly with h-mtTFB1 in 28S mitochondrial ribosomes to augment its 12S rRNA methyltransferase activity, and that together they provide a checkpoint for proper 28S and 55S mitochondrial ribosome assembly. Thus, POLRMT is multi-functional, forming distinct protein complexes that regulate different steps in mitochondrial gene expression, at least one of which does not involve transcription per se. The significance of these results is discussed with regard to the mechanism and regulation of human mitochondrial gene expression and the potential multi-functionality of RNA polymerases in general.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.