ArticleNucleic acids research2013
Using defined finger-finger interfaces as units of assembly for constructing zinc-finger nucleases.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Incorporating AI-optimized zinc finger proteins enhances the efficiencies and targeting ranges of miniature base editors.Nature communications · 2026Article
- A universal deep-learning model for zinc finger design enables transcription factor reprogramming.Nature biotechnology · 2023Article
- Review
- Cleavage-free human genome editing.Molecular therapy : the journal of the American Society of Gene Therapy · 2022Article
- Editing out HIV: application of gene editing technology to achieve functional cure.Retrovirology · 2021Review
- Gene Editing of HIV-1 Co-receptors to Prevent and/or Cure Virus Infection.Frontiers in microbiology · 2018Review
- Article
- DNA-binding-domain fusions enhance the targeting range and precision of Cas9.Nature methods · 2015Article
- A systematic survey of the Cys2His2 zinc finger DNA-binding landscape.Nucleic acids research · 2015Article
- Engineering synthetic TALE and CRISPR/Cas9 transcription factors for regulating gene expression.Methods (San Diego, Calif.) · 2014Article
- The CRISPR/Cas9 System Facilitates Clearance of the Intrahepatic HBV Templates In Vivo.Molecular therapy. Nucleic acids · 2014Article
- The functional significance of common polymorphisms in zinc finger transcription factors.G3 (Bethesda, Md.) · 2014Article
- An improved predictive recognition model for Cys(2)-His(2) zinc finger proteins.Nucleic acids research · 2014Article
- Deep sequencing of large library selections allows computational discovery of diverse sets of zinc fingers that bind common targets.Nucleic acids research · 2014Article
- Light-inducible gene regulation with engineered zinc finger proteins.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Global analysis of Drosophila Cys₂-His₂ zinc finger proteins reveals a multitude of novel recognition motifs and binding determinants.Genome research · 2013Article
- Modeling the specificity of protein-DNA interactions.Quantitative biology (Beijing, China) · 2013Article
- Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Zinc-finger nucleases (ZFNs) have been used for genome engineering in a wide variety of organisms; however, it remains challenging to design effective ZFNs for many genomic sequences using publicly available zinc-finger modules. This limitation is in part because of potential finger-finger incompatibility generated on assembly of modules into zinc-finger arrays (ZFAs). Herein, we describe the validation of a new set of two-finger modules that can be used for building ZFAs via conventional assembly methods or a new strategy-finger stitching-that increases the diversity of genomic sequences targetable by ZFNs. Instead of assembling ZFAs based on units of the zinc-finger structural domain, our finger stitching method uses units that span the finger-finger interface to ensure compatibility of neighbouring recognition helices. We tested this approach by generating and characterizing eight ZFAs, and we found their DNA-binding specificities reflected the specificities of the component modules used in their construction. Four pairs of ZFNs incorporating these ZFAs generated targeted lesions in vivo, demonstrating that stitching yields ZFAs with robust recognition properties.
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Registered trials
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