ArticleBiology of reproduction2013
Locus-specific DNA methylation reprogramming during early porcine embryogenesis.
Article in Biology of reproduction, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- From sperm to pregnancy: insights into paternal regulation of fertility divergence in cattle.The Journal of reproduction and development · 2026Review
- Article
- The Epigenetics of Gametes and Early Embryos and Potential Long-Range Consequences in Livestock Species-Filling in the Picture With Epigenomic Analyses.Frontiers in genetics · 2021Review
- Locus-specific analysis of DNA methylation patterns in cloned and in vitro fertilized porcine embryos.The Journal of reproduction and development · 2020Article
- Follistatin treatment modifies DNA methylation of the CDX2 gene in bovine preimplantation embryos.Molecular reproduction and development · 2020Article
- XIST Derepression in Active X Chromosome Hinders Pig Somatic Cell Nuclear Transfer.Stem cell reports · 2018Article
- Swine models, genomic tools and services to enhance our understanding of human health and diseases.Lab animal · 2017Article
- Cholesterol added prior to vitrification on the cryotolerance of immature and in vitro matured bovine oocytes.PloS one · 2017Article
- Aberrant DNA methylation reprogramming in bovine SCNT preimplantation embryos.Scientific reports · 2016Article
- Differential expression of parental alleles of BRCA1 in human preimplantation embryos.European journal of human genetics : EJHG · 2016Article
- Adult porcine genome-wide DNA methylation patterns support pigs as a biomedical model.BMC genomics · 2015Article
- Epigenetic Modification of Cloned Embryos Improves Nanog Reprogramming in Pigs.Cellular reprogramming · 2015Article
- Oxamflatin treatment enhances cloned porcine embryo development and nuclear reprogramming.Cellular reprogramming · 2015Article
- Epigenetic Modification Agents Improve Gene-Specific Methylation Reprogramming in Porcine Cloned Embryos.PloS one · 2015Article
- Trichostatin A rescues the disrupted imprinting induced by somatic cell nuclear transfer in pigs.PloS one · 2015Article
- Effects of histone deacetylase inhibitor oxamflatin on in vitro porcine somatic cell nuclear transfer embryos.Cellular reprogramming · 2014Article
- Epigenetic modification agents improve genomic methylation reprogramming in porcine cloned embryos.The Journal of reproduction and development · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
During early mammalian embryogenesis, there is a wave of DNA demethylation postfertilization and de novo methylation around implantation. The paternal genome undergoes active DNA demethylation, whereas the maternal genome is passively demethylated after fertilization in most mammals except for sheep and rabbits. However, the emerging genome-wide DNA methylation landscape has revealed a regulatory and locus-specific DNA methylation reprogramming pattern in mammalian preimplantation embryos. Here we optimized a bisulfite sequencing protocol to draw base-resolution DNA methylation profiles of several selected genes in gametes, early embryos, and somatic tissue. We observed locus-specific DNA methylation reprogramming in early porcine embryos. First, some pluripotency genes (POU5F1 and NANOG) followed a typical wave of DNA demethylation and remethylation, whereas CpG-rich regions of SOX2 and CDX2 loci were hypomethylated throughout development. Second, a differentially methylated region of an imprint control region in the IGF2/H19 locus exhibited differential DNA methylation which was maintained in porcine early embryos. Third, a centromeric repeat element retained a moderate DNA methylation level in gametes, early embryos, and somatic tissue. The diverse DNA methylation reprogramming during early embryogenesis is thought to be possibly associated with the multiple functions of DNA methylation in transcriptional regulation, genome stability and genomic imprinting. The latest technology such as oxidative bisulfite sequencing to identify 5-hydroxymethylcytosine will further clarify the DNA methylation reprogramming during porcine embryonic development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.