ArticleJournal of clinical microbiology2013
PCR followed by electrospray ionization mass spectrometry for broad-range identification of fungal pathogens.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 45 citations in OpenAlex.
- Non-Journal of fungi (Basel, Switzerland) · 2023Review
- Rapid detection of Mucorales based on recombinase polymerase amplification and real-time PCR.Frontiers in microbiology · 2023Article
- Microbiological and Molecular Diagnosis of Mucormycosis: From Old to New.Microorganisms · 2021Review
- Evaluation of a PCR-electrospray ionization mass spectrometry platform for detection and identification of fungal pathogens directly from prospectively collected bronchoalveolar lavage specimens.Diagnostic microbiology and infectious disease · 2020Article
- Recent Advances in the Treatment of Scedosporiosis and Fusariosis.Journal of fungi (Basel, Switzerland) · 2018Review
- Assessment of Multi Fragment Melting Analysis System (MFMAS) for the Identification of Food-Borne Yeasts.Current microbiology · 2018Article
- Identification of Microorganisms by High Resolution Tandem Mass Spectrometry with Accurate Statistical Significance.Journal of the American Society for Mass Spectrometry · 2016Article
- Application of Isothermal Amplification Techniques for Identification of Madurella mycetomatis, the Prevalent Agent of Human Mycetoma.Journal of clinical microbiology · 2015Article
- Combat trauma-associated invasive fungal wound infections: epidemiology and clinical classification.Epidemiology and infection · 2015Article
- Purification and characterization of a mycelial catalase from Scedosporium boydii, a useful tool for specific antibody detection in patients with cystic fibrosis.Clinical and vaccine immunology : CVI · 2015Article
- Development of new strategies for early diagnosis of mucormycosis from bench to bedside.Mycoses · 2014Article
- Update on fungal diagnostics.Current infectious disease reports · 2014Article
- Molecular and nonmolecular diagnostic methods for invasive fungal infections.Clinical microbiology reviews · 2014Review
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- Improving the diagnosis of bloodstream infections: PCR coupled with mass spectrometry.BioMed research international · 2014Review
- Review
Corrections and comments
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Authors and funding
15 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Invasive fungal infections are a significant cause of morbidity and mortality among immunocompromised patients. Early and accurate identification of these pathogens is central to direct therapy and to improve overall outcome. PCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was evaluated as a novel means for identification of fungal pathogens. Using a database grounded by 60 ATCC reference strains, a total of 394 clinical fungal isolates (264 molds and 130 yeasts) were analyzed by PCR/ESI-MS; results were compared to phenotypic identification, and discrepant results were sequence confirmed. PCR/ESI-MS identified 81.4% of molds to either the genus or species level, with concordance rates of 89.7% and 87.4%, respectively, to phenotypic identification. Likewise, PCR/ESI-MS was able to identify 98.4% of yeasts to either the genus or species level, agreeing with 100% of phenotypic results at both the genus and species level. PCR/ESI-MS performed best with Aspergillus and Candida isolates, generating species-level identification in 94.4% and 99.2% of isolates, respectively. PCR/ESI-MS is a promising new technology for broad-range detection and identification of medically important fungal pathogens that cause invasive mycoses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.