Evidence map›Paper›PMID 23303494›Full record

ArticleJournal of clinical microbiology2013

Real-time multiplex PCR assay and melting curve analysis for identifying diarrheagenic Escherichia coli.

Tamara B Souza, Diego M Lozer, Sônia M S Kitagawa, Liliana C Spano, Neusa P Silva, Isabel C A Scaletsky

Abstract readComparative StudyEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Association of DiarrheagenicMicroorganisms · 2025
    Article
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  3. Article
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  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Tamara B SouzaDepartamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, Brazil.
Diego M Lozer
Sônia M S Kitagawa
Liliana C Spano
Neusa P Silva
Isabel C A Scaletsky

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A real-time multiplex PCR assay was designed to amplify the virulence genes eae, pEAF, aatA, daaC, elt, est, ipaH, stx(1), and stx(2) for the detection of all diarrheagenic Escherichia coli pathotypes. This assay proved to be more sensitive and rapid than a conventional multiplex PCR for diarrheagenic E. coli isolates from children with diarrhea.

Indexed as

Bacteriological TechniquesChildDiarrheaEscherichia coliEscherichia coli InfectionsEscherichia coli ProteinsGenotypeHumansMolecular Diagnostic TechniquesMultiplex Polymerase Chain ReactionReal-Time Polymerase Chain ReactionSensitivity and SpecificityTransition TemperatureVirulence FactorsEscherichia coli ProteinsVirulence Factors

Identifiers

PMID23303494
PMCPMC3592053

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.