ArticleThe ISME journal2013
A novel approach, based on BLSOMs (Batch Learning Self-Organizing Maps), to the microbiome analysis of ticks.
Article in The ISME journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 118 citations in OpenAlex.
- Characterization of bacterial microbiome and molecular detection of rickettsiosis in free-living ticks (Amblyomma sculptum Berlese, 1888).Scientific reports · 2026Article
- Malagasy flea microbiota results from a combination of vertically transmitted and environmentally acquired microbes.Scientific reports · 2025Article
- Advanced computational tools, artificial intelligence and machine-learning approaches in gut microbiota and biomarker identification.Frontiers in medical technology · 2024Review
- Cross-alteration of murine skin and tick microbiome concomitant with pathogen transmission after Ixodes ricinus bite.Microbiome · 2023Article
- Ticks and Chlamydia-Related Bacteria in Swiss Zoological Gardens Compared to in Contiguous and Distant Control Areas.Microorganisms · 2023Article
- Characterization of the bacterial microbiome of Swedish ticks through 16S rRNA amplicon sequencing of whole ticks and of individual tick organs.Parasites & vectors · 2023Article
- Skin infectome of patients with a tick bite history.Frontiers in cellular and infection microbiology · 2023Article
- Article
- An Exploratory Study on the Microbiome of Northern and Southern Populations ofPathogens (Basel, Switzerland) · 2022Article
- Temporal patterns in Ixodes ricinus microbial communities: an insight into tick-borne microbe interactions.Microbiome · 2021Article
- Molecular Detection and Genotyping ofPathogens (Basel, Switzerland) · 2021Article
- Comparative genomics ofLife science alliance · 2021Article
- Characterization of the bacterial microbiome of Rhipicephalus (Boophilus) microplus collected from Pecari tajacu "Sajino" Madre de Dios, Peru.Scientific reports · 2021Article
- Tick Microbiomes in Neotropical Forest Fragments Are Best Explained by Tick-Associated and Environmental Factors Rather than Host Blood Source.Applied and environmental microbiology · 2021Article
- Interspecific variation and functional traits of the gut microbiome in spiders from the wild: The largest effort so far.PloS one · 2021Article
- Current debates and advances in tick microbiome research.Current research in parasitology & vector-borne diseases · 2021Review
- Behind Taxonomic Variability: The Functional Redundancy in the Tick Microbiome.Microorganisms · 2020Article
- Biotic Factors Influence Microbiota of Nymph Ticks from Vegetation in Sydney, Australia.Pathogens (Basel, Switzerland) · 2020Article
- Analysis of Tick Surface Decontamination Methods.Microorganisms · 2020Article
- Batch-Learning Self-Organizing Map Identifies Horizontal Gene Transfer Candidates and Their Origins in Entire Genomes.Frontiers in microbiology · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ticks transmit a variety of viral, bacterial and protozoal pathogens, which are often zoonotic. The aim of this study was to identify diverse tick microbiomes, which may contain as-yet unidentified pathogens, using a metagenomic approach. DNA prepared from bacteria/archaea-enriched fractions obtained from seven tick species, namely Amblyomma testudinarium, Amblyomma variegatum, Haemaphysalis formosensis, Haemaphysalis longicornis, Ixodes ovatus, Ixodes persulcatus and Ixodes ricinus, was subjected to pyrosequencing after whole-genome amplification. The resulting sequence reads were phylotyped using a Batch Learning Self-Organizing Map (BLSOM) program, which allowed phylogenetic estimation based on similarity of oligonucleotide frequencies, and functional annotation by BLASTX similarity searches. In addition to bacteria previously associated with human/animal diseases, such as Anaplasma, Bartonella, Borrelia, Ehrlichia, Francisella and Rickettsia, BLSOM analysis detected microorganisms belonging to the phylum Chlamydiae in some tick species. This was confirmed by pan-Chlamydia PCR and sequencing analysis. Gene sequences associated with bacterial pathogenesis were also identified, some of which were suspected to originate from horizontal gene transfer. These efforts to construct a database of tick microbes may lead to the ability to predict emerging tick-borne diseases. Furthermore, a comprehensive understanding of tick microbiomes will be useful for understanding tick biology, including vector competency and interactions with pathogens and symbionts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.