ArticleInternational journal of molecular sciences2013
Interaction of proteins identified in human thyroid cells.
Article in International journal of molecular sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
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- High Expression of CISD2 in Relation to Adverse Outcome and Abnormal Immune Cell Infiltration in Glioma.Disease markers · 2022Article
- SCREENED: A Multistage Model of Thyroid Gland Function for Screening Endocrine-Disrupting Chemicals in a Biologically Sex-Specific Manner.International journal of molecular sciences · 2020Review
- Short-Term Microgravity Influences Cell Adhesion in Human Breast Cancer Cells.International journal of molecular sciences · 2019Article
- Fighting Thyroid Cancer with Microgravity Research.International journal of molecular sciences · 2019Review
- In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity.Journal of visualized experiments : JoVE · 2017Article
- Preparation of A Spaceflight: Apoptosis Search in Sutured Wound Healing Models.International journal of molecular sciences · 2017Article
- Impact of Gravity on Thyroid Cells.International journal of molecular sciences · 2017Review
- Identifications of novel mechanisms in breast cancer cells involving duct-like multicellular spheroid formation after exposure to the Random Positioning Machine.Scientific reports · 2016Article
- Simulated Microgravity Using a Rotary Culture System Compromises the In Vitro Development of Mouse Preantral Follicles.PloS one · 2016Article
- The Importance of Caveolin-1 as Key-Regulator of Three-Dimensional Growth in Thyroid Cancer Cells Cultured under Real and Simulated Microgravity Conditions.International journal of molecular sciences · 2015Article
- Mechanisms of three-dimensional growth of thyroid cells during long-term simulated microgravity.Scientific reports · 2015Article
- Common Effects on Cancer Cells Exerted by a Random Positioning Machine and a 2D Clinostat.PloS one · 2015Article
- Growing tissues in real and simulated microgravity: new methods for tissue engineering.Tissue engineering. Part B, Reviews · 2014Review
- Spheroid formation of human thyroid cancer cells under simulated microgravity: a possible role of CTGF and CAV1.Cell communication and signaling : CCS · 2014Article
- Biomarkers for anti-angiogenic therapy in cancer.International journal of molecular sciences · 2013Review
- Interleukin-6 expression under gravitational stress due to vibration and hypergravity in follicular thyroid cancer cells.PloS one · 2013Article
- Genomic approach to identify factors that drive the formation of three-dimensional structures by EA.hy926 endothelial cells.PloS one · 2013Article
- Taking the 3Rs to a higher level: replacement and reduction of animal testing in life sciences in space research.Biotechnology advancesReview
Corrections and comments
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Authors and funding
9 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Influence of gravity forces on the regulation of protein expression by healthy and malignant thyroid cells was studied with the aim to identify protein interactions. Western blot analyses of a limited number of proteins suggested a time-dependent regulation of protein expression by simulated microgravity. After applying free flow isoelectric focusing and mass spectrometry to search for differently expressed proteins by thyroid cells exposed to simulated microgravity for three days, a considerable number of candidates for gravi-sensitive proteins were detected. In order to show how proteins sensitive to microgravity could directly influence other proteins, we investigated all polypeptide chains identified with Mascot scores above 100, looking for groups of interacting proteins. Hence, UniProtKB entry numbers of all detected proteins were entered into the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) and processed. The program indicated that we had detected various groups of interacting proteins in each of the three cell lines studied. The major groups of interacting proteins play a role in pathways of carbohydrate and protein metabolism, regulation of cell growth and cell membrane structuring. Analyzing these groups, networks of interaction could be established which show how a punctual influence of simulated microgravity may propagate via various members of interaction chains.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.