ArticleThe Journal of biological chemistry2013
Novel thylakoid membrane GreenCut protein CPLD38 impacts accumulation of the cytochrome b6f complex and associated regulatory processes.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 26 citations in OpenAlex.
- Functional Genomics Screening inEnvironmental science & technology · 2026Article
- The GreenCut protein TEF6 maintains photosystem II homeostasis under high light by stabilizing FtsH accumulation in Chlamydomonas reinhardtii.Plant communications · 2026Article
- Structure, regulation and assembly of the photosynthetic electron transport chain.Nature reviews. Molecular cell biology · 2025Review
- APX2 Is an Ascorbate Peroxidase-Related Protein that Regulates the Levels of Plastocyanin in Chlamydomonas.Plant & cell physiology · 2024Article
- De-etiolation-induced protein 1 (DEIP1) mediates assembly of the cytochrome bNature communications · 2022Article
- Discovery of photosynthesis genes through whole-genome sequencing of acetate-requiring mutants of Chlamydomonas reinhardtii.PLoS genetics · 2021Article
- Strategies to Study Dark Growth Deficient or Slower Mutants in Chlamydomonas reinhardtii.Methods in molecular biology (Clifton, N.J.) · 2021Article
- A Rubisco-binding protein is required for normal pyrenoid number and starch sheath morphology inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Bilin-Dependent Photoacclimation inThe Plant cell · 2017Article
- Identification of Reference and Biomarker Proteins in Chlamydomonas reinhardtii Cultured under Different Stress Conditions.International journal of molecular sciences · 2017Article
- A repeat protein links Rubisco to form the eukaryotic carbon-concentrating organelle.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- An Indexed, Mapped Mutant Library Enables Reverse Genetics Studies of Biological Processes in Chlamydomonas reinhardtii.The Plant cell · 2016Article
- Critical role of Chlamydomonas reinhardtii ferredoxin-5 in maintaining membrane structure and dark metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- The Use of Contact Mode Atomic Force Microscopy in Aqueous Medium for Structural Analysis of Spinach Photosynthetic Complexes.Plant physiology · 2015Article
- Alternative acetate production pathways in Chlamydomonas reinhardtii during dark anoxia and the dominant role of chloroplasts in fermentative acetate production.The Plant cell · 2014Article
- Inducible Repression of Nuclear-Encoded Subunits of the Cytochrome b6f Complex in Tobacco Reveals an Extraordinarily Long Lifetime of the Complex.Plant physiology · 2014Article
- Proton gradient regulation 5-mediated cyclic electron flow under ATP- or redox-limited conditions: a study of ΔATpase pgr5 and ΔrbcL pgr5 mutants in the green alga Chlamydomonas reinhardtii.Plant physiology · 2014Article
- The GreenCut: re-evaluation of physiological role of previously studied proteins and potential novel protein functions.Photosynthesis research · 2013Review
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9 authors at 3 institutions in 2 countries.
Funding
Abstract
Based on previous comparative genomic analyses, a set of nearly 600 polypeptides was identified that is present in green algae and flowering and nonflowering plants but is not present (or is highly diverged) in nonphotosynthetic organisms. The gene encoding one of these "GreenCut" proteins, CPLD38, is in the same operon as ndhL in most cyanobacteria; the NdhL protein is part of a complex essential for cyanobacterial respiration. A cpld38 mutant of Chlamydomonas reinhardtii does not grow on minimal medium, is high light-sensitive under photoheterotrophic conditions, has lower accumulation of photosynthetic complexes, reduced photosynthetic electron flow to P700(+), and reduced photochemical efficiency of photosystem II (ΦPSII); these phenotypes are rescued by a wild-type copy of CPLD38. Single turnover flash experiments and biochemical analyses demonstrated that cytochrome b6f function was severely compromised, and the levels of transcripts and polypeptide subunits of the cytochrome b6f complex were also significantly lower in the cpld38 mutant. Furthermore, subunits of the cytochrome b6f complex in mutant cells turned over much more rapidly than in wild-type cells. Interestingly, PTOX2 and NDA2, two major proteins involved in chlororespiration, were more than 5-fold higher in mutants relative to wild-type cells, suggesting a shift in the cpld38 mutant from photosynthesis toward chlororespiratory metabolism, which is supported by experiments that quantify the reduction state of the plastoquinone pool. Together, these findings support the hypothesis that CPLD38 impacts the stability of the cytochrome b6f complex and possibly plays a role in balancing redox inputs to the quinone pool from photosynthesis and chlororespiration.
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