ArticleThe Journal of biological chemistry2013
Formation and fate of a complete 31-protein RNA polymerase II transcription preinitiation complex.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Regulation of the RNA polymerase II pre-initiation complex by its associated coactivators.Nature reviews. Genetics · 2023Review
- Structural basis of a transcription pre-initiation complex on a divergent promoter.Molecular cell · 2023Article
- Structural visualization of de novo transcription initiation by Saccharomyces cerevisiae RNA polymerase II.Molecular cell · 2022Article
- NSUN6, an RNA methyltransferase of 5-mC controls glioblastoma response to temozolomide (TMZ) via NELFB and RPS6KB2 interaction.Cancer biology & therapy · 2021Article
- Dynamics of RNA polymerase II and elongation factor Spt4/5 recruitment during activator-dependent transcription.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A Single-Molecule Surface-Based Platform to Detect the Assembly and Function of the Human RNA Polymerase II Transcription Machinery.Structure (London, England : 1993) · 2020Article
- Recent advances in understanding RNA polymerase II structure and function.Faculty reviews · 2020Review
- The capping enzyme facilitates promoter escape and assembly of a follow-on preinitiation complex for reinitiation.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- The TAFs of TFIID Bind and Rearrange the Topology of the TATA-LessInternational journal of molecular sciences · 2019Article
- In vitro reconstitution of yeast RNA polymerase II transcription initiation with high efficiency.Methods (San Diego, Calif.) · 2019Article
- Def1 interacts with TFIIH and modulates RNA polymerase II transcription.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Real-time observation of polymerase-promoter contact remodeling during transcription initiation.Nature communications · 2017Article
- Chromatin potentiates transcription.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Single-Molecule Real-Time 3D Imaging of the Transcription Cycle by Modulation Interferometry.Cell · 2016Article
- Structure of an RNA polymerase II preinitiation complex.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Article
- Uncoupling Promoter Opening from Start-Site Scanning.Molecular cell · 2015Article
- Transcription factors TFIIF and TFIIS promote transcript elongation by RNA polymerase II by synergistic and independent mechanisms.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The RNA polymerase II preinitiation complex. Through what pathway is the complex assembled?Transcription · 2014Review
- Structural insights into transcription initiation by RNA polymerase II.Trends in biochemical sciences · 2013Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Whereas individual RNA polymerase II (pol II)-general transcription factor (GTF) complexes are unstable, an assembly of pol II with six GTFs and promoter DNA could be isolated in abundant homogeneous form. The resulting complete pol II transcription preinitiation complex (PIC) contained equimolar amounts of all 31 protein components. An intermediate in assembly, consisting of four GTFs and promoter DNA, could be isolated and supplemented with the remaining components for formation of the PIC. Nuclease digestion and psoralen cross-linking mapped the PIC between positions -70 and -9, centered on the TATA box. Addition of ATP to the PIC resulted in quantitative conversion to an open complex, which retained all 31 proteins, contrary to expectation from previous studies. Addition of the remaining NTPs resulted in run-off transcription, with an efficiency that was promoter-dependent and was as great as 17.5% with the promoters tested.
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