ArticleThe Journal of biological chemistry2013
In situ determination of manganese(II) speciation in Deinococcus radiodurans by high magnetic field EPR: detection of high levels of Mn(II) bound to proteins.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 44 citations in OpenAlex.
- Rising from the fragments: a multi-factorial regulatory framework of DNA damage response in Deinococcus radiodurans.Extremophiles : life under extreme conditions · 2026Review
- Metals in Motion: Understanding Labile Metal Pools in Bacteria.Biochemistry · 2025Review
- A water-soluble, cell-permeable Mn(ii) sensor enables visualization of manganese dynamics in live mammalian cells.Chemical science · 2024Article
- A novel ionizing radiation-induced small RNA, DrsS, promotes the detoxification of reactive oxygen species inApplied and environmental microbiology · 2024Article
- Article
- Structural and Functional Characterization of the Holliday Junction Resolvase RuvC fromMicroorganisms · 2022Article
- In vitro reconstitution of an efficient nucleotide excision repair system using mesophilic enzymes from Deinococcus radiodurans.Communications biology · 2022Article
- In-Cell Characterization of the Stable Tyrosyl Radical in E. coli Ribonucleotide Reductase Using Advanced EPR Spectroscopy.Angewandte Chemie (International ed. in English) · 2021Article
- Intracellular Metal Speciation inACS infectious diseases · 2020Article
- Gre-family factors modulate DNA damage sensing byRNA biology · 2019Article
- The interplay between Mn and Fe in Deinococcus radiodurans triggers cellular protection during paraquat-induced oxidative stress.Scientific reports · 2019Article
- Metal homeostasis and resistance in bacteria.Nature reviews. Microbiology · 2017Review
- Regulation of transcriptional pausing through the secondary channel of RNA polymerase.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Manganese homeostasis and utilization in pathogenic bacteria.Molecular microbiology · 2015Review
- Dysregulation of transition metal ion homeostasis is the molecular basis for cadmium toxicity in Streptococcus pneumoniae.Nature communications · 2015Article
- Article
- Specificity of metal sensing: iron and manganese homeostasis in Bacillus subtilis.The Journal of biological chemistry · 2014Review
- Responses of Mn2+ speciation in Deinococcus radiodurans and Escherichia coli to γ-radiation by advanced paramagnetic resonance methods.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High magnetic field high frequency electron paramagnetic resonance techniques were used to measure in situ Mn(II) speciation in Deinococcus radiodurans, a radiation-resistant bacteria capable of accumulating high concentrations of Mn(II). It was possible to identify and quantify the evolution of Mn(II) species in intact cells at various stages of growth. Aside from water, 95-GHz high field electron nuclear double resonance showed that the Mn(II) ions are bound to histidines and phosphate groups, mostly from fructose-1,6-bisphosphate but also inorganic phosphates and nucleotides. During stationary growth phase, 285-GHz continuous wave EPR measurements showed that histidine is the most common ligand to Mn(II) and that significant amounts of cellular Mn(II) in D. radiodurans are bound to peptides and proteins. As much as 40% of the total Mn(II) was in manganese superoxide dismutase, and it is this protein and not smaller manganese complexes, as has been suggested recently, that is probably the primary defense against superoxide.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.