ArticleClinical and vaccine immunology : CVI2013
Enhanced detection of host response antibodies to Borrelia burgdorferi using immuno-PCR.
Article in Clinical and vaccine immunology : CVI, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Suppression of host humoral immunity byInfection and immunity · 2024Article
- Lyme borreliosis diagnosis: state of the art of improvements and innovations.BMC microbiology · 2023Review
- Endogenous Linear Plasmids lp28-4 and lp25 Are Required for Infectivity and Restriction Protection in the Lyme Disease Spirochete Borrelia mayonii.Infection and immunity · 2023Article
- SARS-CoV-2 Serosurveys: How Antigen, Isotype and Threshold Choices Affect the Outcome.The Journal of infectious diseases · 2023Article
- Is Immuno-PCR Better than ELISA Test for Detection of Toxoplasma gondii IgG Antibody?Acta parasitologica · 2022Article
- A murine model of Lyme disease demonstrates that Borrelia burgdorferi colonizes the dura mater and induces inflammation in the central nervous system.PLoS pathogens · 2021Article
- A Practical Approach to the Diagnosis of Lyme Borreliosis: From Clinical Heterogeneity to Laboratory Methods.Frontiers in medicine · 2020Review
- Article
- Recent strategies for the diagnosis of early Lyme disease.Science progress · 2018Review
- Immuno-PCR technology for detection of natural human antibodies against LeGlycoconjugate journal · 2017Article
- The Past, Present, and (Possible) Future of Serologic Testing for Lyme Disease.Journal of clinical microbiology · 2016Review
- Evaluation of Selected Borrelia burgdorferi lp54 Plasmid-Encoded Gene Products Expressed during Mammalian Infection as Antigens To Improve Serodiagnostic Testing for Early Lyme Disease.Clinical and vaccine immunology : CVI · 2015Article
- Simple objective detection of human lyme disease infection using immuno-PCR and a single recombinant hybrid antigen.Clinical and vaccine immunology : CVI · 2014Article
- Protein detection through different platforms of immuno-loop-mediated isothermal amplification.Nanoscale research letters · 2013Article
- In vivo expression technology identifies a novel virulence factor critical for Borrelia burgdorferi persistence in mice.PLoS pathogens · 2013Article
- Lyme Disease: An Overview.Indian dermatology online journalReview
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Authors and funding
3 authors.
Funding
Abstract
Lyme disease is the fastest-growing zoonotic disease in North America. Current methods for detection of Borrelia burgdorferi infection are challenged by analysis subjectivity and standardization of antigen source. In the present study, we developed an immuno-PCR (iPCR)-based approach employing recombinant in vivo-expressed B. burgdorferi antigens for objective detection of a host immune response to B. burgdorferi infection. iPCR is a liquid-phase protein detection method that combines the sensitivity of PCR with the specificity and versatility of immunoassay-based protocols. Use of magnetic beads coated with intact spirochetes provided effective antigen presentation and allowed detection of host-generated antibodies in experimentally infected mice at day 11 postinoculation, whereas host-generated antibodies were detected at day 14 by enzyme-linked immunosorbent assay (ELISA) and day 21 by immunoblotting. Furthermore, magnetic beads coated with recombinant B. burgdorferi in vivo-expressed antigen OspC or BmpA demonstrated positive detection of host-generated antibodies in mice at day 7 postinoculation with markedly increased iPCR signals above the background, with the quantification cycle (C(q)) value for each sample minus the mean background C(q) plus 3 standard deviations (ΔC(q)) being 4 to 10, whereas ΔC(q) was 2.5 for intact spirochete-coated beads. iPCR demonstrated a strong correlation (Spearman rank correlation = 0.895, P < 0.0001) with a commercial ELISA for detection of host antibodies in human Lyme disease patient sera using the B. burgdorferi VlsE C6 peptide. In addition, iPCR showed potential applicability for direct detection of spirochetes in blood. The results presented here indicate that our iPCR assay has the potential to provide an objective format that can be used for sensitive detection of multiple host response antibodies and isotypes to B. burgdorferi infection.
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