ArticleBMC plant biology2013
Functional genomics of a generalist parasitic plant: laser microdissection of host-parasite interface reveals host-specific patterns of parasite gene expression.
Article in BMC plant biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 88 citations in OpenAlex.
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- Seed metabolites headstart haustoriogenesis and potentiate aggressiveness of parasitic weeds.Science advances · 2025Article
- IAA-Mediated Haustorium Formation inPlants (Basel, Switzerland) · 2025Article
- inDAGO: a user-friendly interface for seamless dual and bulk RNA-Seq analysis.Frontiers in bioinformatics · 2025Article
- Unveiling the mechanisms driving the rapid growth ofFrontiers in plant science · 2025Article
- Pectin Remodeling and Involvement of AtPME3 in the Parasitic Plant-Plant Interaction,Plants (Basel, Switzerland) · 2024Article
- Comparative secretome analysis of Striga and Cuscuta species identifies candidate virulence factors for two evolutionarily independent parasitic plant lineages.BMC plant biology · 2024Article
- Integrated Transcriptome and Proteome Analysis Reveals That Cell Wall Activity AffectsPlants (Basel, Switzerland) · 2024Article
- Refining dual RNA-seq mapping: sequential and combined approaches in host-parasitic plant dynamics.Frontiers in plant science · 2024Article
- Pectin modifications promote haustoria development in the parasitic plant Phtheirospermum japonicum.Plant physiology · 2023Article
- Genomic and Epigenomic Mechanisms of the Interaction between Parasitic and Host Plants.International journal of molecular sciences · 2023Review
- Genome-enabled discovery of candidate virulence loci in Striga hermonthica, a devastating parasite of African cereal crops.The New phytologist · 2022Article
- Article
- An artificial host system enables the obligate parasite Cuscuta campestris to grow and reproduce in vitro.Plant physiology · 2022Article
- Genomic reconfiguration in parasitic plants involves considerable gene losses alongside global genome size inflation and gene births.Plant physiology · 2021Article
- Molecular actors of seed germination and haustoriogenesis in parasitic weeds.Plant physiology · 2021Article
- WARPP-web application for the research of parasitic plants.Plant physiology · 2021Article
- Subtilase activity in intrusive cells mediates haustorium maturation in parasitic plants.Plant physiology · 2021Article
- Integrated analyses of the transcriptome and small RNA of the hemiparasitic plant Monochasma savatieri before and after establishment of parasite-host association.BMC plant biology · 2021Article
- Management of Infection by Parasitic Weeds: A Review.Plants (Basel, Switzerland) · 2020Review
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Authors and funding
11 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOrobanchaceae is the only plant family with members representing the full range of parasitic lifestyles plus a free-living lineage sister to all parasitic lineages, Lindenbergia. A generalist member of this family, and an important parasitic plant model, Triphysaria versicolor regularly feeds upon a wide range of host plants. Here, we compare de novo assembled transcriptomes generated from laser micro-dissected tissues at the host-parasite interface to uncover details of the largely uncharacterized interaction between parasitic plants and their hosts.
resultsThe interaction of Triphysaria with the distantly related hosts Zea mays and Medicago truncatula reveals dramatic host-specific gene expression patterns. Relative to above ground tissues, gene families are disproportionally represented at the interface including enrichment for transcription factors and genes of unknown function. Quantitative Real-Time PCR of a T. versicolor β-expansin shows strong differential (120x) upregulation in response to the monocot host Z. mays; a result that is concordant with our read count estimates. Pathogenesis-related proteins, other cell wall modifying enzymes, and orthologs of genes with unknown function (annotated as such in sequenced plant genomes) are among the parasite genes highly expressed by T. versicolor at the parasite-host interface.
conclusionsLaser capture microdissection makes it possible to sample the small region of cells at the epicenter of parasite host interactions. The results of our analysis suggest that T. versicolor's generalist strategy involves a reliance on overlapping but distinct gene sets, depending upon the host plant it is parasitizing. The massive upregulation of a T. versicolor β-expansin is suggestive of a mechanism for parasite success on grass hosts. In this preliminary study of the interface transcriptomes, we have shown that T. versicolor, and the Orobanchaceae in general, provide excellent opportunities for the characterization of plant genes with unknown functions.
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