ArticlePLoS neglected tropical diseases2013
Non-invasive in vivo study of the Trypanosoma vivax infectious process consolidates the brain commitment in late infections.
Article in PLoS neglected tropical diseases, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 37 citations in OpenAlex.
- Noninvasive, Fluorescence-Based Detection of Perturbations in the Thiol-Redox Homeostasis of Bloodstream Trypanosoma brucei brucei.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Investigation ofCell surface (Amsterdam, Netherlands) · 2023Article
- Pathogenicity and virulence of African trypanosomes: From laboratory models to clinically relevant hosts.Virulence · 2023Review
- Cloning and Characterization ofInternational journal of molecular sciences · 2023Article
- Vivaxin genes encode highly immunogenic, non-variant antigens on the Trypanosoma vivax cell-surface.PLoS neglected tropical diseases · 2022Article
- An Update on African Trypanocide Pharmaceutics and Resistance.Frontiers in veterinary science · 2022Review
- Article
- Reliable, scalable functional genetics in bloodstream-form Trypanosoma congolense in vitro and in vivo.PLoS pathogens · 2021Article
- To the Skin and Beyond: The Immune Response to African Trypanosomes as They Enter and Exit the Vertebrate Host.Frontiers in immunology · 2020Review
- Recent advances in functional research in Giardia intestinalis.Advances in parasitology · 2020Review
- Route of inoculation influences Trypanosoma congolense and Trypanosoma brucei brucei virulence in Swiss white mice.PloS one · 2019Article
- Comparative experimental studies onHeliyon · 2018Article
- In-vivo monitoring of infectious diseases in living animals using bioluminescence imaging.Virulence · 2018Review
- Transcriptomic Profiling of High-DensityFrontiers in cellular and infection microbiology · 2017Article
- The animal trypanosomiases and their chemotherapy: a review.Parasitology · 2016Review
- The Cyclical Development ofFrontiers in cellular and infection microbiology · 2016Article
- Comparative clinico-pathological observations in young Zebu (Bos indicus) cattle experimentally infected with Trypanosoma vivax isolates from tsetse infested and non-tsetse areas of Northwest Ethiopia.BMC veterinary research · 2015Article
- Phylogenetic and syntenic data support a single horizontal transference to a Trypanosoma ancestor of a prokaryotic proline racemase implicated in parasite evasion from host defences.Parasites & vectors · 2015Article
- A new experimental model for assessing drug efficacy against Trypanosoma cruzi infection based on highly sensitive in vivo imaging.Journal of biomolecular screening · 2015Review
- A panel of Trypanosoma brucei strains tagged with blue and red-shifted luciferases for bioluminescent imaging in murine infection models.PLoS neglected tropical diseases · 2014Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trypanosoma vivax, one of the leading parasites responsible for Animal African Trypanosomosis (Nagana), is generally cyclically transmitted by Glossina spp. but in areas devoid of the tsetse flies in Africa or in Latin American countries is mechanically transmitted across vertebrate hosts by other haematophagous insects, including tabanids. We followed on from our recent studies on the maintenance of this parasite in vivo and in vitro, and its genetic manipulation, by constructing a West African IL1392 T. vivax strain that stably expresses firefly luciferase and is fully virulent for immunocompetent mice. We report here on a study where murine infection with this strain was monitored in vivo using a non-invasive method. Study findings fully support the use of this strain in the assessment of parasite dynamics in vivo since a strong correlation was found between whole body light emission measured over the course of the infection and parasitemia determined microscopically. In addition, parasitemia and survival rates were very similar for mice infected by the intraperitoneal and sub-cutaneous routes, except for a longer prepatent period following sub-cutaneous inoculation with the parasite. Our results clearly show that when administered by the subcutaneous route, the parasite is retained few days in the skin close to the inoculation site where it multiplies before passing into the bloodstream. Ex vivo bioluminescence analyses of organs isolated from infected mice corroborated our previous histopathological observations with parasite infiltration into spleen, liver and lungs. Finally, our study reinforces previous observations on the presence of the parasite in the central nervous system and consequently the brain commitment in the very late phases of the experimental infection.
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