ArticlePloS one2013
Single-cell and population NF-κB dynamic responses depend on lipopolysaccharide preparation.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Sulfation Rewrites the Biological Profile of Dietary Flavonoids: Implications for Stability, Redox Signaling, and Inflammation.Food science & nutrition · 2026Article
- Elevated pre-activation basal level of nuclear NF-κB in native macrophages accelerates LPS-induced translocation of cytosolic NF-κB into the cell nucleus.Scientific reports · 2019Article
- Combinatorial processing of bacterial and host-derived innate immune stimuli at the single-cell level.Molecular biology of the cell · 2019Article
- Immune Functional Assays, From Custom to Standardized Tests for Precision Medicine.Frontiers in immunology · 2018Review
- Subcellular western blotting of single cells.Microsystems & nanoengineering · 2017Article
- Cyclic stretch induced IL-33 production through HMGB1/TLR-4 signaling pathway in murine respiratory epithelial cells.PloS one · 2017Article
- Article
- Controversial Role of Toll-like Receptor 4 in Adult Stem Cells.Stem cell reviews and reports · 2015Review
- In vitro γ-ray-induced inflammatory response is dominated by culturing conditions rather than radiation exposures.Scientific reports · 2015Article
- Single-cell variation leads to population invariance in NF-κB signaling dynamics.Molecular biology of the cell · 2015Article
- Switching of the relative dominance between feedback mechanisms in lipopolysaccharide-induced NF-κB signaling.Science signaling · 2014Article
- Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundLipopolysaccharide (LPS), found in the outer membrane of gram-negative bacteria, elicits a strong response from the transcription factor family Nuclear factor (NF)-κB via Toll-like receptor (TLR) 4. The cellular response to lipopolysaccharide varies depending on the source and preparation of the ligand, however. Our goal was to compare single-cell NF-κB dynamics across multiple sources and concentrations of LPS. METHODOLOGY/PRINCIPAL
findingsUsing live-cell fluorescence microscopy, we determined the NF-κB activation dynamics of hundreds of single cells expressing a p65-dsRed fusion protein. We used computational image analysis to measure the nuclear localization of the fusion protein in the cells over time. The concentration range spanned up to nine orders of magnitude for three E. coli LPS preparations. We find that the LPS preparations induce markedly different responses, even accounting for potency differences. We also find that the ability of soluble TNF receptor to affect NF-κB dynamics varies strikingly across the three preparations. CONCLUSIONS/SIGNIFICANCE: Our work strongly suggests that the cellular response to LPS is highly sensitive to the source and preparation of the ligand. We therefore caution that conclusions drawn from experiments using one preparation may not be applicable to LPS in general.
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