ArticlePloS one2012
Butyrate produced by commensal bacteria potentiates phorbol esters induced AP-1 response in human intestinal epithelial cells.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 82 citations in OpenAlex.
- Using Volatile Organic Compounds to Investigate the Effect of Oral Iron Supplementation on the Human Intestinal Metabolome.Molecules (Basel, Switzerland) · 2020Trial
- Microbial short chain fatty acids: Effective histone deacetylase inhibitors in immune regulation (Review).International journal of molecular medicine · 2026Review
- Recent advances in the positive role ofFrontiers in immunology · 2026Review
- The intricate interplay of microbial metabolomics and carcinogenesis: a spotlight on mechanistic pathways, clinical implications and methodological challenges.Frontiers in cellular and infection microbiology · 2026Review
- Estrobolome dysbiosis in canine mammary gland tumors:Frontiers in microbiology · 2026Article
- The Effect of Apple and Pear Cultivars on In Vitro Fermentation with Human Faecal Microbiota.Microorganisms · 2025Article
- AgeAnnoMO: a knowledgebase of multi-omics annotation for animal aging.Nucleic acids research · 2024Article
- An Insight into Functional Metagenomics: A High-Throughput Approach to Decipher Food-Microbiota-Host Interactions in the Human Gut.International journal of molecular sciences · 2023Review
- Regulation of dietary fiber on intestinal microorganisms and its effects on animal health.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2023Review
- Review
- Polysaccharides as Potential Anti-tumor Biomacromolecules -A Review.Frontiers in nutrition · 2022Review
- Managing Gut Microbiota through In Ovo Nutrition Influences Early-Life Programming in Broiler Chickens.Animals : an open access journal from MDPI · 2021Review
- Soluble Dietary Fiber, One of the Most Important Nutrients for the Gut Microbiota.Molecules (Basel, Switzerland) · 2021Review
- Comparison of Fecal Microbiota of Horses Suffering from Atypical Myopathy and Healthy Co-Grazers.Animals : an open access journal from MDPI · 2021Article
- Analysis of Cow-Calf Microbiome Transfer Routes and Microbiome Diversity in the Newborn Holstein Dairy Calf Hindgut.Frontiers in nutrition · 2021Article
- Positive Synergistic Effects of Quercetin and Rice Bran on Human Gut Microbiota ReducesFrontiers in microbiology · 2021Article
- Short-Chain Fatty Acids (Except Hexanoic Acid) Lower NF-kB Transactivation, Which Rescues Inflammation-Induced Decreased Apolipoprotein A-I Transcription in HepG2 Cells.International journal of molecular sciences · 2020Article
- Microbiota-dependent and -independent effects of dietary fibre on human health.British journal of pharmacology · 2020Review
- The small chain fatty acid butyrate antagonizes the TCR-stimulation-induced metabolic shift in murine epidermal gamma delta T cells.EXCLI journal · 2020Article
- Microbiome as an Immunological Modifier.Methods in molecular biology (Clifton, N.J.) · 2020Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human intestine is a balanced ecosystem well suited for bacterial survival, colonization and growth, which has evolved to be beneficial both for the host and the commensal bacteria. Here, we investigated the effect of bacterial metabolites produced by commensal bacteria on AP-1 signaling pathway, which has a plethora of effects on host physiology. Using intestinal epithelial cell lines, HT-29 and Caco-2, stably transfected with AP-1-dependent luciferase reporter gene, we tested the effect of culture supernatant from 49 commensal strains. We observed that several bacteria were able to activate the AP-1 pathway and this was correlated to the amount of short chain fatty acids (SCFAs) produced. Besides being a major source of energy for epithelial cells, SCFAs have been shown to regulate several signaling pathways in these cells. We show that propionate and butyrate are potent activators of the AP-1 pathway, butyrate being the more efficient of the two. We also observed a strong synergistic activation of AP-1 pathway when using butyrate with PMA, a PKC activator. Moreover, butyrate enhanced the PMA-induced expression of c-fos and ERK1/2 phosphorylation, but not p38 and JNK. In conclusion, we showed that SCFAs especially butyrate regulate the AP-1 signaling pathway, a feature that may contribute to the physiological impact of the gut microbiota on the host. Our results provide support for the involvement of butyrate in modulating the action of PKC in colon cancer cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.