ArticlePloS one2012
Identification and functional demonstration of miRNAs in the fungus Cryptococcus neoformans.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 69 citations in OpenAlex.
- Discovering the hidden function in fungal genomes.Nature communications · 2024Review
- Challenges and Opportunities Arising from Host-International journal of molecular sciences · 2024Review
- The Biological Roles ofBiomolecules · 2023Review
- RNA-Seq, Bioinformatic Identification of Potential MicroRNA-like Small RNAs in the Edible MushroomInternational journal of molecular sciences · 2022Article
- Discovery of microRNA-like Small RNAs in Pathogenic Plant FungusInternational journal of molecular sciences · 2022Article
- Comparative analysis of RNA enrichment methods for preparation of Cryptococcus neoformans RNA sequencing libraries.G3 (Bethesda, Md.) · 2021Article
- Identification of virulence associated milRNAs and their bidirectional targets in Rhizoctonia solani and maize during infection.BMC plant biology · 2021Article
- Identification and physiological function of one microRNA (Mycoscience · 2021Article
- The Evolutionary Significance of RNAi in the Fungal Kingdom.International journal of molecular sciences · 2020Review
- Identification and characterization of Dicer-like genes in leaf rust pathogen (Puccinia triticina) of wheat.Functional & integrative genomics · 2020Article
- Transcriptomic Analysis of Extracellular RNA Governed by the Endocytic Adaptor Protein Cin1 ofFrontiers in cellular and infection microbiology · 2020Article
- Article
- Discovery and profiling of small RNAs from Puccinia triticina by deep sequencing and identification of their potential targets in wheat.Functional & integrative genomics · 2019Article
- Extracellular Vesicle-Mediated RNA Release inmSphere · 2019Article
- Label-free Quantitative Analysis of Protein Expression Alterations in miR-26a-Knockout HeLa Cells using SWATH-MS Technology.Scientific reports · 2019Article
- Comprehensive analysis of full genome sequence andIMA fungus · 2019Article
- Integrated microRNA and mRNA analysis in the pathogenic filamentous fungus Trichophyton rubrum.BMC genomics · 2018Article
- Comparative genome-wide analysis of extracellular small RNAs from the mucormycosis pathogen Rhizopus delemar.Scientific reports · 2018Article
- Dicer-Like Proteins Regulate Sexual Development via the Biogenesis of Perithecium-Specific MicroRNAs in a Plant Pathogenic FungusFrontiers in microbiology · 2018Article
- Cell Wall Synthesis, Development of Hyphae and Metabolic Pathways Are Processes Potentially Regulated by MicroRNAs Produced Between the Morphological Stages ofFrontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
microRNAs (miRNAs), endogenous posttranscriptional repressors by base-pairing of their cognate mRNAs in plants and animals, have mostly been thought lost in the kingdom of fungi. Here, we report the identification of miRNAs from the fungus Cryptococcus neoformans. With bioinformatics and Northern blotting approaches, we found that these miRNAs and their hairpin precursors were present in this fungus. The size of miR1 and miR2 is 22 nt and 18 nt, respectively. The precursors are about ∼70 nt in length that is close to mammalian pre-miRNAs. Characteristic features of miRNAs are also found in miR1/2. We demonstrated that the identified miRNAs, miR1 and miR2, caused transgene silencing via the canonical RNAi pathway. Bioinformantics analysis helps to reveal a number of identical sequences of the miR1/2 in transposable elements (TEs) and pseudogenes, prompting us to think that fungal miRNAs might be involved in the regulation of the activity of transposons and the expression of pseudogenes. This study identified functional miRNAs in C. neoformans, and sheds light on the diversity and evolutionary origin of eukaryotic miRNAs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.