ArticlePloS one2012
Characterization of an acute muscle contraction model using cultured C2C12 myotubes.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers, 1 of them a synthesis that pooled it.
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Who cites it
63 citing papers in PubMed, 1 synthesis or guideline pooled it, 117 citations in OpenAlex.
- Characteristics of the Protocols Used in Electrical Pulse Stimulation of Cultured Cells for Mimicking In Vivo Exercise: A Systematic Review, Meta-Analysis, and Meta-Regression.International journal of molecular sciences · 2022Pooled it
- Small Extracellular Vesicle Release Following Electrical Pulse Stimulation of C2C12 Myotubes: Effects on microRNA Cargo and Myoblast Migration and Differentiation.International journal of molecular sciences · 2026Article
- Fast-swimming biohybrid OstraBot with self-trained high-strength muscles.Nature communications · 2026Article
- Short-duration electrical pulse stimulation induces myokines and mitochondrial adaptations in C2C12 myotubes.Journal of muscle research and cell motility · 2026Article
- In Vitro and Clinical Evaluation of the Anti-Wrinkle Efficacy of Medipep-6PN, a Novel Peptide Identified by Phage Display.International journal of molecular sciences · 2026Article
- Electrical pulse stimulation reflecting the episodic nature of real-life exercise modulates metabolic and secretory profile of primary human myotubes.FEBS open bio · 2026Article
- Development of Electric Field Stimulation (EFS)-Mediated Skeletal Muscle Training with iPSCs.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Modulating MyoD1 dosage activates alternate cell fate beyond myogenic differentiation.bioRxiv : the preprint server for biology · 2025Article
- Effect of transforming growth factor beta receptor I inhibitors on myotube formation in vitro.Scientific reports · 2025Article
- Piezo1 channels enhance anabolic signaling activation induced by electrical stimulation of cultured myotubes.FEBS open bio · 2025Article
- Article
- Stimulated C2C12 Myotube Headspace Volatile Organic Compound Analysis.Molecules (Basel, Switzerland) · 2024Article
- Electrical Pulse Stimulation Protects C2C12 Myotubes against Hydrogen Peroxide-Induced Cytotoxicity via Nrf2/Antioxidant Pathway.Antioxidants (Basel, Switzerland) · 2024Article
- Virus-free transfection, transient expression, and purification of human cardiac myosin in mammalian muscle cells for biochemical and biophysical assays.Scientific reports · 2023Article
- Establishment of a system evaluating the contractile force of electrically stimulated myotubes from wrinkles formed on elastic substrate.Scientific reports · 2022Article
- The Effect of Deoxycholic Acid on Chitosan-Enabled Matrices for Tissue Scaffolding and Injectable Nanogels.Gels (Basel, Switzerland) · 2022Article
- Involvement of Parkin-mediated mitophagy in the pathogenesis of chronic obstructive pulmonary disease-related sarcopenia.Journal of cachexia, sarcopenia and muscle · 2022Article
- A Piezo1/KLF15/IL-6 axis mediates immobilization-induced muscle atrophy.The Journal of clinical investigation · 2022Article
- Bio-printing of aligned GelMa-based cell-laden structure for muscle tissue regeneration.Bioactive materials · 2022Article
- ViaFuse: Fiji macros to calculate skeletal muscle cell viability and fusion index.Skeletal muscle · 2021Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
A cultured C2C12 myotube contraction system was examined for application as a model for acute contraction-induced phenotypes of skeletal muscle. C2C12 myotubes seeded into 4-well rectangular plates were placed in a contraction system equipped with a carbon electrode at each end. The myotubes were stimulated with electric pulses of 50 V at 1 Hz for 3 ms at 997-ms intervals. Approximately 80% of the myotubes were observed to contract microscopically, and the contractions lasted for at least 3 h with electrical stimulation. Calcium ion (Ca²⁺) transient evoked by the electric pulses was detected fluorescently with Fluo-8. Phosphorylation of protein kinase B/Akt (Akt), 5' AMP-activated protein kinase (AMPK), p38 mitogen-activated protein kinase (p38), and c-Jun NH2-terminal kinase (JNK)1/2, which are intracellular signaling proteins typically activated in exercised/contracted skeletal muscle, was observed in the electrically stimulated C2C12 myotubes. The contractions induced by the electric pulses increased glucose uptake and depleted glycogen in the C2C12 myotubes. C2C12 myotubes that differentiated after exogenous gene transfection by a lipofection or an electroporation method retained their normal contractile ability by electrical stimulation. These findings show that our C2C12 cell contraction system reproduces the muscle phenotypes that arise invivo (exercise), in situ (hindlimb muscles in an anesthetized animal), and invitro (dissected muscle tissues in incubation buffer) by acute muscle contraction, demonstrating that the system is applicable for the analysis of intracellular events evoked by acute muscle contraction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.