ArticlePloS one2012
DNA builds and strengthens the extracellular matrix in Myxococcus xanthus biofilms by interacting with exopolysaccharides.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 82 citations in OpenAlex.
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- DNA at the center of mammalian innate immune recognition of bacterial biofilms.Trends in immunology · 2024Review
- A unique combination of natural fatty acids fromFrontiers in cellular and infection microbiology · 2024Article
- Glycosyl hydrolase fromBiofilm · 2023Article
- Isolation and Staining Reveal the Presence of Extracellular DNA in Marine Gel Particles.Gels (Basel, Switzerland) · 2023Article
- Effect of Calcium Ion Supplementation on Oral Microbial Composition and Biofilm Formation In Vitro.Microorganisms · 2022Article
- The structural role of bacterial eDNA in the formation of biofilm streamers.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Theranostic nanoplatforms of emodin-chitosan with blue laser light on enhancing the anti-biofilm activity of photodynamic therapy against Streptococcus mutans biofilms on the enamel surface.BMC microbiology · 2022Article
- Physicochemical and Biological Insights Into the Molecular Interactions Between Extracellular DNA and Exopolysaccharides inFrontiers in microbiology · 2022Article
- Bacteriophage-Mediated Control of Biofilm: A Promising New Dawn for the Future.Frontiers in microbiology · 2022Review
- Extracellular DNA (eDNA). A Major Ubiquitous Element of the Bacterial Biofilm Architecture.International journal of molecular sciences · 2021Review
- The Chemical Structure Properties and Promoting Biofilm Activity of Exopolysaccharide Produced byFrontiers in microbiology · 2021Article
- Characterization of biofilm formed by multidrug resistantSaudi journal of biological sciences · 2020Article
- Bifidobacterial biofilm formation is a multifactorial adaptive phenomenon in response to bile exposure.Scientific reports · 2020Article
- Novel Bacterial Diversity and Fragmented eDNA Identified in Hyperbiofilm-Forming Pseudomonas aeruginosa Rugose Small Colony Variant.iScience · 2020Article
- Interplay of mesoscale physics and agent-like behaviors in the parallel evolution of aggregative multicellularity.EvoDevo · 2020Review
- The extracellular DNA lattice of bacterial biofilms is structurally related to Holliday junction recombination intermediates.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 3 countries.
Funding
Abstract
One intriguing discovery in modern microbiology is the extensive presence of extracellular DNA (eDNA) within biofilms of various bacterial species. Although several biological functions have been suggested for eDNA, including involvement in biofilm formation, the detailed mechanism of eDNA integration into biofilm architecture is still poorly understood. In the biofilms formed by Myxococcus xanthus, a Gram-negative soil bacterium with complex morphogenesis and social behaviors, DNA was found within both extracted and native extracellular matrices (ECM). Further examination revealed that these eDNA molecules formed well organized structures that were similar in appearance to the organization of exopolysaccharides (EPS) in ECM. Biochemical and image analyses confirmed that eDNA bound to and colocalized with EPS within the ECM of starvation biofilms and fruiting bodies. In addition, ECM containing eDNA exhibited greater physical strength and biological stress resistance compared to DNase I treated ECM. Taken together, these findings demonstrate that DNA interacts with EPS and strengthens biofilm structures in M. xanthus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.