ArticlePloS one2012
Efficient measurement of opsonising antibodies to Plasmodium falciparum merozoites.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Vaccine-induced mouse antibodies targeting Plasmodium falciparum PfVFT antigen inhibit blood stages through multiple mechanisms.NPJ vaccines · 2026Article
- Naturally acquired antibodies from Beninese infants promote Plasmodium falciparum merozoite-phagocytosis by human blood leukocytes: implications for control of asymptomatic malaria infections.Malaria journal · 2022Article
- Improvement of the antibody-dependent respiratory burst assay for assessing protective immune responses to malaria.Open biology · 2022Article
- GMZ2 Vaccine-Induced Antibody Responses, Naturally Acquired Immunity and the Incidence of Malaria in Burkinabe Children.Frontiers in immunology · 2022Article
- Neutrophils dominate in opsonic phagocytosis of P. falciparum blood-stage merozoites and protect against febrile malaria.Communications biology · 2021Article
- Peripheral Merozoite Surface Proteins Are Targets of Naturally Acquired Immunity against Malaria in both India and Ghana.Infection and immunity · 2020Article
- Article
- Molecular Characterization and Immuno-Reactivity Patterns of a NovelFrontiers in cellular and infection microbiology · 2020Article
- A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry.Memorias do Instituto Oswaldo Cruz · 2019Article
- Patterns of protective associations differ for antibodies to P. falciparum-infected erythrocytes and merozoites in immunity against malaria in children.European journal of immunology · 2017Article
- An Opsonic Phagocytosis Assay for Plasmodium falciparum Sporozoites.Clinical and vaccine immunology : CVI · 2017Article
- Recent insights into humoral immunity targeting Plasmodium falciparum and Plasmodium vivax malaria.International journal for parasitology · 2017Review
- Merozoite Antigens of Plasmodium falciparum Elicit Strain-Transcending Opsonizing Immunity.Infection and immunity · 2016Article
- Standardization of the antibody-dependent respiratory burst assay with human neutrophils and Plasmodium falciparum malaria.Scientific reports · 2015Article
- Activated Brain Endothelial Cells Cross-Present Malaria Antigen.PLoS pathogens · 2015Article
- High yield purification of Plasmodium falciparum merozoites for use in opsonizing antibody assays.Journal of visualized experiments : JoVE · 2014Article
- Opsonic phagocytosis of Plasmodium falciparum merozoites: mechanism in human immunity and a correlate of protection against malaria.BMC medicine · 2014Article
- Assessment of antibody-dependent respiratory burst activity from mouse neutrophils on Plasmodium yoelii malaria challenge outcome.Journal of leukocyte biology · 2014Article
- Evaluating controlled human malaria infection in Kenyan adults with varying degrees of prior exposure to Plasmodium falciparum using sporozoites administered by intramuscular injection.Frontiers in microbiology · 2014Article
- Challenges of assessing the clinical efficacy of asexual blood-stage Plasmodium falciparum malaria vaccines.Human vaccines & immunotherapeutics · 2013Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundAntibodies targeting merozoites are important in protection from malaria. Therefore, merozoite surface proteins are attractive vaccine candidates. There is a need for robust functional assays to investigate mechanisms of acquired immunity and vaccine efficacy. To date, the study of merozoite phagocytosis has been confounded by the complexity and variability of in vitro assays. METHODOLOGY/PRINCIPAL
findingsWe have developed a new flow cytometry-based merozoite phagocytosis assay. An optimized merozoite preparation technique produced high yields of merozoites separated from haemozoin. Phagocytosis by the undifferentiated THP-1 monocytic cell line was mediated only by Fc Receptors, and was therefore ideal for studying opsonising antibody responses. The assay showed robust phagocytosis with highly diluted immune sera and strong inter-assay correlation. The assay effectively measured differences in opsonisation-dependent phagocytosis among individuals. CONCLUSIONS/SIGNIFICANCE: This highly reproducible assay has potential applications in assessing the role of opsonic phagocytosis in naturally acquired immunity and vaccine trials.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.