ArticlePLoS genetics2013
Transcriptional dynamics elicited by a short pulse of notch activation involves feed-forward regulation by E(spl)/Hes genes.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- The Drosophila histone variant H2Av facilitates Notch signaling activity in a two-tier regulatory fashion.Cell communication and signaling : CCS · 2025Article
- Notch-Dependent Expression of theGenes · 2024Article
- Article
- Regulation of immune cell development, differentiation and function by stromal Notch ligands.Current opinion in cell biology · 2023Review
- A spatiotemporal Notch interaction map from plasma membrane to nucleus.Science signaling · 2023Article
- Jag1-Notch cis-interaction determines cell fate segregation in pancreatic development.Nature communications · 2023Article
- Notch-dependent DNA cis-regulatory elements and their dose-dependent control of C. elegans stem cell self-renewal.Development (Cambridge, England) · 2022Article
- Image-Based Single-Molecule Analysis of Notch-Dependent Transcription in Its Natural Context.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The Membrane-Bound Notch Regulator Mnr Supports Notch Cleavage and Signaling Activity inBiomolecules · 2021Article
- Enhancers with cooperative Notch binding sites are more resistant to regulation by the Hairless co-repressor.PLoS genetics · 2021Article
- Phospho-Site Mutations in Transcription Factor Suppressor of Hairless Impact Notch Signaling Activity During Hematopoiesis inFrontiers in cell and developmental biology · 2021Article
- Article
- Optogenetic inhibition of Delta reveals digital Notch signalling output during tissue differentiation.EMBO reports · 2019Article
- Article
- Article
- Learning Causal Biological Networks With the Principle of Mendelian Randomization.Frontiers in genetics · 2019Article
- Long-term live imaging of theeLife · 2018Article
- Variant cell cycles regulated by Notch signaling control cell size and ensure a functional blood-brain barrier.Development (Cambridge, England) · 2018Article
- Article
- Integration of Drosophila and Human Genetics to Understand Notch Signaling Related Diseases.Advances in experimental medicine and biology · 2018Review
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8 authors.
Funding
Abstract
Dynamic activity of signaling pathways, such as Notch, is vital to achieve correct development and homeostasis. However, most studies assess output many hours or days after initiation of signaling, once the outcome has been consolidated. Here we analyze genome-wide changes in transcript levels, binding of the Notch pathway transcription factor, CSL [Suppressor of Hairless, Su(H), in Drosophila], and RNA Polymerase II (Pol II) immediately following a short pulse of Notch stimulation. A total of 154 genes showed significant differential expression (DE) over time, and their expression profiles stratified into 14 clusters based on the timing, magnitude, and direction of DE. E(spl) genes were the most rapidly upregulated, with Su(H), Pol II, and transcript levels increasing within 5-10 minutes. Other genes had a more delayed response, the timing of which was largely unaffected by more prolonged Notch activation. Neither Su(H) binding nor poised Pol II could fully explain the differences between profiles. Instead, our data indicate that regulatory interactions, driven by the early-responding E(spl)bHLH genes, are required. Proposed cross-regulatory relationships were validated in vivo and in cell culture, supporting the view that feed-forward repression by E(spl)bHLH/Hes shapes the response of late-responding genes. Based on these data, we propose a model in which Hes genes are responsible for co-ordinating the Notch response of a wide spectrum of other targets, explaining the critical functions these key regulators play in many developmental and disease contexts.
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