ArticlePLoS genetics2012
Evidence for the robustness of protein complexes to inter-species hybridization.
Article in PLoS genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- Evolutionary causes and consequences of gene duplication.Nature reviews. Genetics · 2026Review
- Correlated Evolution Drives Structural Convergence of Interacting Proteins.Journal of molecular evolution · 2026Article
- Multiple intermolecular interactions facilitate rapid evolution of essential genes.Nature ecology & evolution · 2023Article
- Proteotoxicity caused by perturbed protein complexes underlies hybrid incompatibility in yeast.Nature communications · 2022Article
- Are transient protein-protein interactions more dispensable?PLoS computational biology · 2022Article
- Yeast proteins do not practice social distancing as species hybridize.Current genetics · 2021Review
- Interspecific hybridization as a driver of fungal evolution and adaptation.Nature reviews. Microbiology · 2021Review
- Frequent Assembly of Chimeric Complexes in the Protein Interaction Network of an Interspecies Yeast Hybrid.Molecular biology and evolution · 2021Article
- Protein Complexes Form a Basis for Complex Hybrid Incompatibility.Frontiers in genetics · 2021Review
- Genomic instability in an interspecific hybrid of the genusMicrobial genomics · 2020Article
- Speciation and the developmental alarm clock.eLife · 2020Review
- Estimating dispensable content in the human interactome.Nature communications · 2019Article
- Overexpression ofMicroorganisms · 2019Article
- Identification of the fitness determinants of budding yeast on a natural substrate.The ISME journal · 2017Article
- Systematic Protein-Protein Interaction Analysis Reveals Intersubcomplex Contacts in the Nuclear Pore Complex.Molecular & cellular proteomics : MCP · 2016Article
- An inter-species protein-protein interaction network across vast evolutionary distance.Molecular systems biology · 2016Article
- A Cross-Species Study of PI3K Protein-Protein Interactions Reveals the Direct Interaction of P85 and SHP2.Scientific reports · 2016Article
- Genome-wide protein-protein interaction screening by protein-fragment complementation assay (PCA) in living cells.Journal of visualized experiments : JoVE · 2015Article
- A test of double interspecific introgression of nucleoporin genes in Drosophila.G3 (Bethesda, Md.) · 2014Article
- Ribosome profiling reveals post-transcriptional buffering of divergent gene expression in yeast.Genome research · 2014Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite the tremendous efforts devoted to the identification of genetic incompatibilities underlying hybrid sterility and inviability, little is known about the effect of inter-species hybridization at the protein interactome level. Here, we develop a screening platform for the comparison of protein-protein interactions (PPIs) among closely related species and their hybrids. We examine in vivo the architecture of protein complexes in two yeast species (Saccharomyces cerevisiae and Saccharomyces kudriavzevii) that diverged 5-20 million years ago and in their F1 hybrids. We focus on 24 proteins of two large complexes: the RNA polymerase II and the nuclear pore complex (NPC), which show contrasting patterns of molecular evolution. We found that, with the exception of one PPI in the NPC sub-complex, PPIs were highly conserved between species, regardless of protein divergence. Unexpectedly, we found that the architecture of the complexes in F1 hybrids could not be distinguished from that of the parental species. Our results suggest that the conservation of PPIs in hybrids likely results from the slow evolution taking place on the very few protein residues involved in the interaction or that protein complexes are inherently robust and may accommodate protein divergence up to the level that is observed among closely related species.
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