ArticlePLoS pathogens2012
The Calmodulin-like calcium binding protein EhCaBP3 of Entamoeba histolytica regulates phagocytosis and is involved in actin dynamics.
Article in PLoS pathogens, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 55 citations in OpenAlex.
- Ancestral TALE homeobox protein transcription factor regulates actin dynamics and cellular activities of protozoan parasite Entamoeba invadens.Molecular microbiology · 2024Article
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- Transcriptomic profiling of "brain-eating amoeba"Frontiers in cellular and infection microbiology · 2024Article
- Molecular interplays of theFrontiers in cellular and infection microbiology · 2022Review
- Trogocytosis in Unicellular Eukaryotes.Cells · 2021Review
- An atypical EhGEF regulates phagocytosis in Entamoeba histolytica through EhRho1.PLoS pathogens · 2021Article
- The Dichloromethane Fraction ofFrontiers in cellular and infection microbiology · 2021Article
- ArfX2 GTPase Regulates Trafficking From the Trans-Golgi to Lysosomes and Is Necessary for Liver Abscess Formation in the Protozoan ParasiteFrontiers in cellular and infection microbiology · 2021Article
- Structural and functional diversity of Entamoeba histolytica calcium-binding proteins.Biophysical reviews · 2020Review
- Rab GTPases take centre stage in understandingSmall GTPases · 2020Review
- Entamoeba histolytica and pathogenesis: A calcium connection.PLoS pathogens · 2020Review
- EhC2B, a C2 domain-containing protein, promotes erythrophagocytosis in Entamoeba histolytica via actin nucleation.PLoS pathogens · 2020Article
- Apoptotic mimicry as a strategy for the establishment of parasitic infections: parasite- and host-derived phosphatidylserine as key molecule.Cell communication and signaling : CCS · 2020Review
- Lipids inFrontiers in cellular and infection microbiology · 2020Review
- Revisiting Drug Development Against the Neglected Tropical Disease, Amebiasis.Frontiers in cellular and infection microbiology · 2020Review
- Uncovering the Cyclic AMP Signaling Pathway of the Protozoan ParasiteFrontiers in cellular and infection microbiology · 2020Article
- EhP3, a homolog of 14-3-3 family of protein participates in actin reorganization and phagocytosis in Entamoeba histolytica.PLoS pathogens · 2019Article
- Membrane associated proteins of two Trichomonas gallinae clones vary with the virulence.PloS one · 2019Article
- Stress-induced nuclear depletion ofThe Journal of biological chemistry · 2018Article
- AGC family kinase 1 participates in trogocytosis but not in phagocytosis in Entamoeba histolytica.Nature communications · 2017Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phagocytosis is required for proliferation and pathogenesis of Entamoeba histolytica and erythrophagocytosis is considered to be a marker of invasive amoebiasis. Ca²⁺ has been found to play a central role in the process of phagocytosis. However, the molecular mechanisms and the signalling mediated by Ca²⁺ still remain largely unknown. Here we show that Calmodulin-like calcium binding protein EhCaBP3 of E. histolytica is directly involved in disease pathomechanism by its capacity to participate in cytoskeleton dynamics and scission machinery during erythrophagocytosis. Using imaging techniques EhCaBP3 was found in phagocytic cups and newly formed phagosomes along with actin and myosin IB. In vitro studies confirmed that EhCaBP3 directly binds actin, and affected both its polymerization and bundling activity. Moreover, it also binds myosin 1B in the presence of Ca²⁺. In cells where EhCaBP3 expression was down regulated by antisense RNA, the level of RBC uptake was reduced, myosin IB was found to be absent at the site of pseudopod cup closure and the time taken for phagocytosis increased, suggesting that EhCaBP3 along with myosin 1B mediate the closure of phagocytic cups. Experiments with EhCaBP3 mutant defective in Ca²⁺-binding showed that Ca²⁺ binding is required for phagosome formation. Liposome binding assay revealed that EhCaBP3 recruitment and enrichment to membrane is independent of any cellular protein as it binds directly to phosphatidylserine. Taken together, our results suggest a novel pathway mediating phagocytosis in E. histolytica, and an unusual mechanism of modulation of cytoskeleton dynamics by two calcium binding proteins, EhCaBP1 and EhCaBP3 with mostly non-overlapping functions.
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