ArticlePoultry science2013
Characterization of bacteriophages virulent for Clostridium perfringens and identification of phage lytic enzymes as alternatives to antibiotics for potential control of the bacterium.
Article in Poultry science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
- Isolation, Identification, and Prophylactic Evaluation of a Newly IsolatedMicroorganisms · 2026Article
- Morphological and complete genome analysis of a novelFrontiers in pharmacology · 2026Article
- A Comprehensive Review of the Application of Bacteriophages Against Enteric Bacterial Infection in Poultry: Current Status, Challenges, and Future Prospects.Antibiotics (Basel, Switzerland) · 2025Review
- New Perspectives in the Fight Against Multidrug-Resistant Bacteria: The Potential of Endolysin Biocomposites.Antibiotics (Basel, Switzerland) · 2025Review
- Bacteriophage therapy for controlling poultry production problems.Iranian journal of veterinary research · 2025Review
- Genome MLST scheme for tracing genetic diversity and multidrug resistance of food animal-derivedCurrent research in food science · 2025Article
- AmiP from hyperthermophilic Thermus parvatiensis prophage is a thermoactive and ultrathermostable peptidoglycan lytic amidase.Protein science : a publication of the Protein Society · 2023Article
- A Broad-Spectrum Phage Endolysin (LysCP28) Able to Remove Biofilms and InactivateFoods (Basel, Switzerland) · 2023Article
- Article
- Phage derived lytic peptides, a secret weapon againstFrontiers in medicine · 2022Article
- Bacteriophage mediated control of necrotic enteritis caused by C. perfringens in broiler chickens.Veterinary research communications · 2021Article
- Assessment of Immune Response and Efficacy of Essential Oils Application on Controlling Necrotic Enteritis Induced byMolecules (Basel, Switzerland) · 2021Article
- Phage therapy as a revolutionary medicine against Gram-positive bacterial infections.Beni-Suef University journal of basic and applied sciences · 2021Review
- Phage lysin that specifically eliminates Clostridium botulinum Group I cells.Scientific reports · 2020Article
- Phages in Anaerobic Systems.Viruses · 2020Review
- The Use of Bacteriophages in the Poultry Industry.Animals : an open access journal from MDPI · 2020Review
- Optimized production of a biologically active Clostridium perfringens glycosyl hydrolase phage endolysin PlyCP41 in plants using virus-based systemic expression.BMC biotechnology · 2019Article
- Capsular Polysaccharide Is a Receptor of aViruses · 2019Article
- Clostridium perfringens Virulent Bacteriophage CPS2 and Its Thermostable Endolysin LysCPS2.Viruses · 2018Article
- Biochemical and in silico evaluation of recombinant E. coli aminopeptidase and in vitro processed human interferon α-2b.Turkish journal of biology = Turk biyoloji dergisi · 2018Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
There has been a resurgent interest in the use of bacteriophages or their gene products to control bacterial pathogens as alternatives to currently used antibiotics. Clostridium perfringens is a gram-positive, spore-forming anaerobic bacterium that plays a significant role in human foodborne disease as well as non-foodborne human, animal, and avian diseases. Countries that have complied with the ban on antimicrobial growth promoters in feeds have reported increased incidences of C. perfringens-associated diseases in poultry. To address these issues, new antimicrobial agents, putative lysins encoded by the genomes of bacteriophages, are being identified in our laboratory. Poultry intestinal material, soil, sewage, and poultry processing drainage water were screened for virulent bacteriophages that could lyse C. perfringens and produce clear plaques in spot assays. Bacteriophages were isolated that had long noncontractile tails, members of the family Siphoviridae, and with short noncontractile tails, members of the family Podoviridae. Several bacteriophage genes were identified that encoded N-acetylmuramoyl-l-alanine amidases, lysozyme-endopeptidases, and a zinc carboxypeptidase domain that has not been previously reported in viral genomes. Putative phage lysin genes (ply) were cloned and expressed in Escherichia coli. The recombinant lysins were amidases capable of lysing both parental phage host strains of C. perfringens as well as other strains of the bacterium in spot and turbidity reduction assays, but did not lyse any clostridia beyond the species. Consequently, bacteriophage gene products could eventually be used to target bacterial pathogens, such as C. perfringens via a species-specific strategy, to control animal and human diseases without having deleterious effects on beneficial probiotic bacteria.
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Registered trials
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