ArticleProceedings of the National Academy of Sciences of the United States of America2013
Microfluidic experiments reveal that antifreeze proteins bound to ice crystals suffice to prevent their growth.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 169 citations in OpenAlex.
- Antifreeze Protein for Freeze-Thaw Durability Enhancement of Cement Mortar: Effects and Action Analysis.Materials (Basel, Switzerland) · 2026Article
- How to measure the activity of antifreeze proteins?Biophysical reviews · 2025Review
- Convergent evolution of type I antifreeze proteins from four different progenitors in response to global cooling.BMC molecular and cell biology · 2024Article
- Protein-Decorated Reverse Osmosis Membranes with High Gypsum Scaling Resistance.ACS environmental Au · 2024Article
- Psychrotrophic Bacteria Threatening the Safety of Animal-Derived Foods: Characteristics, Contamination, and Control Strategies.Food science of animal resources · 2024Review
- On the engulfment of antifreeze proteins by ice.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Extended Temperature Range of the Ice-Binding Protein Activity.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- The Nanoliter Osmometer: Thermal Hysteresis Measurement.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Enhanced cold tolerance mechanisms inFrontiers in microbiology · 2024Article
- Divergent Mechanisms of Ice Growth Inhibition by Antifreeze Proteins.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Investigating the Interaction Between Ice-Binding Proteins and Ice Surfaces Using Microfluidic Devices and Cold Stages.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Article
- Nanoscopy of single antifreeze proteins reveals that reversible ice binding is sufficient for ice recrystallization inhibition but not thermal hysteresis.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Natural Cryoprotective and Cytoprotective Agents in Cryopreservation: A Focus on Melatonin.Molecules (Basel, Switzerland) · 2022Review
- Antifreeze Proteins: Novel Applications and Navigation towards Their Clinical Application in Cryobanking.International journal of molecular sciences · 2022Review
- Structural diversity of marine anti-freezing proteins, properties and potential applications: a review.Bioresources and bioprocessing · 2022Review
- The role of antifreeze glycoprotein (AFGP) and polyvinyl alcohol/polyglycerol (X/Z-1000) as ice modulators during partial freezing of rat livers.Frontiers in physics · 2022Article
- IND-enzymes: a repository for hydrolytic enzymes derived from thermophilic and psychrophilic bacterial species with potential industrial usage.Extremophiles : life under extreme conditions · 2021Article
- Article
- Antifreeze Proteins and Their Practical Utilization in Industry, Medicine, and Agriculture.Biomolecules · 2020Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
Antifreeze proteins (AFPs) are a subset of ice-binding proteins that control ice crystal growth. They have potential for the cryopreservation of cells, tissues, and organs, as well as for production and storage of food and protection of crops from frost. However, the detailed mechanism of action of AFPs is still unclear. Specifically, there is controversy regarding reversibility of binding of AFPs to crystal surfaces. The experimentally observed dependence of activity of AFPs on their concentration in solution appears to indicate that the binding is reversible. Here, by a series of experiments in temperature-controlled microfluidic devices, where the medium surrounding ice crystals can be exchanged, we show that the binding of hyperactive Tenebrio molitor AFP to ice crystals is practically irreversible and that surface-bound AFPs are sufficient to inhibit ice crystal growth even in solutions depleted of AFPs. These findings rule out theories of AFP activity relying on the presence of unbound protein molecules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.