ArticleGenome research2013
RNA-seq-based mapping and candidate identification of mutations from forward genetic screens.
Article in Genome research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
58 citing papers in PubMed.
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- An initial HOPS-mediated fusion event is critical for autophagosome transport initiation from the axon terminal.Autophagy · 2024Article
- Forward genetics combined with unsupervised classifications identified zebrafish mutants affecting biliary system formation.Developmental biology · 2024Article
- Maternal regulation of the vertebrate oocyte-to-embryo transition.PLoS genetics · 2024Article
- Exploring hematopoiesis in zebrafish using forward genetic screening.Experimental & molecular medicine · 2024Review
- The secreted neuronal signal Spock1 promotes blood-brain barrier development.Developmental cell · 2023Article
- Transcript profiling of hairy vetch (Vicia villosa Roth) identified interesting genes for seed dormancy.The plant genome · 2023Article
- Review
- NudC regulated Lis1 stability is essential for the maintenance of dynamic microtubule ends in axon terminals.iScience · 2022Article
- Utility of Triti-Map for bulk-segregated mapping of causal genes and regulatory elements in Triticeae.Plant communications · 2022Article
- Connexinplexity: the spatial and temporal expression of connexin genes during vertebrate organogenesis.G3 (Bethesda, Md.) · 2022Article
- Translational relevance of forward genetic screens in animal models for the study of psychiatric disease.Neuroscience and biobehavioral reviews · 2022Review
- Allelically and Differentially Expressed Genes After Infection of Edwardsiella ictaluri in Channel Catfish as Determined by Bulk Segregant RNA-Seq.Marine biotechnology (New York, N.Y.) · 2022Article
- Allele-specific gene expression can underlie altered transcript abundance in zebrafish mutants.eLife · 2022Article
- ThemicroPublication biology · 2022Article
- Direct-TRI: High-throughput RNA-extracting Method for All Stages of Zebrafish Development.Bio-protocol · 2021Article
- NCK-associated protein 1 like (nckap1l) minor splice variant regulates intrahepatic biliary network morphogenesis.PLoS genetics · 2021Article
- Haematopoietic stem cell-dependent Notch transcription is mediated by p53 through the Histone chaperone Supt16h.Nature cell biology · 2020Article
- Next Generation Sequencing Based Forward Genetic Approaches for Identification and Mapping of Causal Mutations in Crop Plants: A Comprehensive Review.Plants (Basel, Switzerland) · 2020Review
- Transcriptomic profiling and discovery of key genes involved in adventitious root formation from green cuttings of highbush blueberry (Vaccinium corymbosum L.).BMC plant biology · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Forward genetic screens have elucidated molecular pathways required for innumerable aspects of life; however, identifying the causal mutations from such screens has long been the bottleneck in the process, particularly in vertebrates. We have developed an RNA-seq-based approach that identifies both the region of the genome linked to a mutation and candidate lesions that may be causal for the phenotype of interest. We show that our method successfully identifies zebrafish mutations that cause nonsense or missense changes to codons, alter transcript splicing, or alter gene expression levels. Furthermore, we develop an easily accessible bioinformatics pipeline allowing for implementation of all steps of the method. Overall, we show that RNA-seq is a fast, reliable, and cost-effective method to map and identify mutations that will greatly facilitate the power of forward genetics in vertebrate models.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.