ArticleThe EMBO journal2013
A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy.
Article in The EMBO journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 140 papers.
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Who cites it
140 citing papers in PubMed.
- [Cystic lymphangioma of the penis in an adult: A case report].Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2026Article
- Lymphatic Endothelial Cells in Health and Disease.MedComm · 2026Review
- Decreased potential for lymphatic vessel generation is a hallmark of early diagnosed arterial hypertension and can be reversed by treatment with angiotensin converting enzyme inhibitors.Scientific reports · 2026Article
- Insights into KIF11 pathogenesis in microcephaly-lymphedema-chorioretinopathy syndrome from a lymphatic perspective.JCI insight · 2026Article
- Meningeal macrophages regulate fibroblasts to influence meningeal lymphatic function following traumatic brain injury.Theranostics · 2026Article
- APOA1 binding protein promotes lymphatic cell fate and lymphangiogenesis by relieving caveolae-mediated inhibition of VEGFR3 signaling.Nature communications · 2025Article
- Nanobody Immunolabelling and three-dimensional imaging reveals spatially restricted LYVE1 expression by kidney lymphatic vessels in mice.Journal of nanobiotechnology · 2025Article
- Meningeal lymphatic drainage: novel insights into central nervous system disease.Signal transduction and targeted therapy · 2025Review
- Direct specification of lymphatic endothelium from mesenchymal progenitors.Nature cardiovascular research · 2025Article
- Whole-brain 3D imaging of dopaminergic neurons and glial cells in the mouse model of Parkinson's disease induced by 6-OHDA.Frontiers in aging neuroscience · 2025Article
- Paraxial mesoderm as a direct gateway to lymphatic endothelial cells.Nature cardiovascular research · 2025Article
- Neutrophils in tumor- and inflammation-induced lymphangiogenesis.International journal of biological sciences · 2025Review
- Mesenchymal Osr1+ cells regulate embryonic lymphatic vessel formation.Development (Cambridge, England) · 2024Article
- Central conducting lymphatic anomaly: from bench to bedside.The Journal of clinical investigation · 2024Review
- Lymphatic vessel: origin, heterogeneity, biological functions, and therapeutic targets.Signal transduction and targeted therapy · 2024Review
- Erythematous capillary-lymphatic malformations mimicking blood vascular anomalies.JCI insight · 2023Article
- Fluid flow to mimic organ function in 3DAPL bioengineering · 2023Review
- [Genetics, diagnostics and clinical presentation of primary lymphoedema].Dermatologie (Heidelberg, Germany) · 2023Review
- Single-cell analysis of lymphatic endothelial cell fate specification and differentiation during zebrafish development.The EMBO journal · 2023Article
- GATA2 regulates blood/lymph separation in a platelet-dependent and lymphovenous valve-independent manner.Microcirculation (New York, N.Y. : 1994) · 2023Article
80 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During mammalian development, a subpopulation of endothelial cells in the cardinal vein (CV) expresses lymphatic-specific genes and subsequently develops into the first lymphatic structures, collectively termed as lymph sacs. Budding, sprouting and ballooning of lymphatic endothelial cells (LECs) have been proposed to underlie the emergence of LECs from the CV, but the exact mechanisms of lymph vessel formation remain poorly understood. Applying selective plane illumination-based ultramicroscopy to entire wholemount-immunostained mouse embryos, we visualized the complete developing vascular system with cellular resolution. Here, we report emergence of the earliest detectable LECs as strings of loosely connected cells between the CV and superficial venous plexus. Subsequent aggregation of LECs resulted in formation of two distinct, previously unidentified lymphatic structures, the dorsal peripheral longitudinal lymphatic vessel (PLLV) and the ventral primordial thoracic duct (pTD), which at later stages formed a direct contact with the CV. Providing new insights into their function, we found vascular endothelial growth factor C (VEGF-C) and the matrix component CCBE1 indispensable for LEC budding and migration. Altogether, we present a significantly more detailed view and novel model of early lymphatic development.
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