ArticleNature communications2013
A thermoresponsive and chemically defined hydrogel for long-term culture of human embryonic stem cells.
Article in Nature communications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 123 citations in OpenAlex.
- Airway basal cells from human-induced pluripotent stem cells: a new frontier in cystic fibrosis research.Frontiers in cell and developmental biology · 2024Article
- Downstream bioprocessing of human pluripotent stem cell-derived therapeutics.Engineering in life sciences · 2022Review
- A chemically-defined plastic scaffold for the xeno-free production of human pluripotent stem cells.Scientific reports · 2022Article
- Passive myocardial mechanical properties: meaning, measurement, models.Biophysical reviews · 2021Review
- Discovery of hemocompatible bacterial biofilm-resistant copolymers.Biomaterials · 2020Article
- ToF-SIMS and Machine Learning for Single-Pixel Molecular Discrimination of an Acrylate Polymer Microarray.Analytical chemistry · 2020Article
- Miniaturized high-throughput synthesis and screening of responsive hydrogels using nanoliter compartments.Materials today. Bio · 2020Article
- Glioma progression and recurrence involving maintenance and expansion strategies of glioma stem cells by organizing self-advantageous niche microenvironments.Inflammation and regeneration · 2020Review
- Probing the mechanism for hydrogel-based stasis induction in human pluripotent stem cells: is the chemical functionality of the hydrogel important?Chemical science · 2019Article
- Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array.Journal of visualized experiments : JoVE · 2018Article
- Why the impact of mechanical stimuli on stem cells remains a challenge.Cellular and molecular life sciences : CMLS · 2018Review
- New substrates for stem cell control.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs.Scientific reports · 2017Article
- Versatile synthetic alternatives to Matrigel for vascular toxicity screening and stem cell expansion.Nature biomedical engineering · 2017Article
- Application of Targeted Molecular and Material Property Optimization to Bacterial Attachment-Resistant (Meth)acrylate Polymers.Biomacromolecules · 2016Article
- Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion.Biomaterials science · 2016Article
- Pluripotent stem cell derived hepatocytes: using materials to define cellular differentiation and tissue engineering.Journal of materials chemistry. B · 2016Review
- Polymer microarray technology for stem cell engineering.Acta biomaterialia · 2016Review
- Mucin-Inspired Thermoresponsive Synthetic Hydrogels Induce Stasis in Human Pluripotent Stem Cells and Human Embryos.ACS central science · 2016Article
- Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
16 authors at 4 institutions in 3 countries.
Funding
Abstract
Cultures of human embryonic stem cell typically rely on protein matrices or feeder cells to support attachment and growth, while mechanical, enzymatic or chemical cell dissociation methods are used for cellular passaging. However, these methods are ill defined, thus introducing variability into the system, and may damage cells. They also exert selective pressures favouring cell aneuploidy and loss of differentiation potential. Here we report the identification of a family of chemically defined thermoresponsive synthetic hydrogels based on 2-(diethylamino)ethyl acrylate, which support long-term human embryonic stem cell growth and pluripotency over a period of 2-6 months. The hydrogels permitted gentle, reagent-free cell passaging by virtue of transient modulation of the ambient temperature from 37 to 15 °C for 30 min. These chemically defined alternatives to currently used, undefined biological substrates represent a flexible and scalable approach for improving the definition, efficacy and safety of human embryonic stem cell culture systems for research, industrial and clinical applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.