Evidence map›Paper›PMID 23299740›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2013

Quantitative and qualitative preparations of bacterial outer membrane vesicles.

Halima Chutkan, Ian Macdonald, Andrew Manning, Meta J Kuehn

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.

0numbers the graph read from it
0cells of the map it votes in
85citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

85 citing papers in PubMed.

  1. Article
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  3. Review
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  8. Article
  9. Violacein-Loaded Outer Membrane Vesicles fromACS biomaterials science & engineering · 2025
    Article
  10. Review
  11. Article
  12. Microbiology spectrum · 2025
    Article
  13. Article
  14. Article
  15. Article
  16. Article
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  18. Article
  19. Article
  20. Article

25 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Halima ChutkanDepartment of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC, USA.
Ian Macdonald
Andrew Manning
Meta J Kuehn

Funding

Enterotoxin targeting and delivery mechanismsR01AI064464 · NIAID · DUKE UNIVERSITY · PI KUEHN, META J · 2006 to 2010
$1.5M
Triggers and Consequences of Bacterial Envelope StressR01GM099471 · NIGMS · DUKE UNIVERSITY · PI KUEHN, META J · 2012 to 2015
$1.1M
Biogenesis and Function of Bacterial Outer Membrane VesiclesR01AI079068 · NIAID · DUKE UNIVERSITY · PI KUEHN, META J · 2009 to 2010
$750k
NIAID NIH HHS R01 AI064464NIAID NIH HHS R01AI064464NIAID NIH HHS R01 AI079068NIAID NIH HHS R01AI079068NIGMS NIH HHS R01 GM099471NIGMS NIH HHS R01GM099471-01
6 · The paper itself

Abstract

Gram-negative bacterial outer membrane vesicle production and function have been studied using a variety of quantitative and qualitative methods. These types of analyses can be hampered by the use of impure vesicle preparations. Here we describe a set of techniques that are useful for the quantitative analysis of vesicle production and for preparative yields of highly purified vesicles for studies of vesicle function or composition. Procedures and advice are also included for the purification of vesicles from encapsulated and low-yield strains.

Indexed as

Bacterial Outer Membrane ProteinsElectrophoresis, Polyacrylamide GelGram-Negative BacteriaMicroscopy, ElectronBacterial Outer Membrane Proteins

Identifiers

PMID23299740
PMCPMC4317262

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.