ArticleMethods in molecular biology (Clifton, N.J.)2013
Chromatographic analysis of the Escherichia coli polysialic acid capsule.
Article in Methods in molecular biology (Clifton, N.J.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed, 5 citations in OpenAlex.
- Review
- Comparative genomic analysis of the flagellin glycosylation island of the Gram-positive thermophile Geobacillus.BMC genomics · 2016Article
- Unexpected Diversity of Escherichia coli Sialate O-Acetyl Esterase NanS.Journal of bacteriology · 2016Article
- Observational
- Unified theory of bacterial sialometabolism: how and why bacteria metabolize host sialic acids.ISRN microbiology · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Polysialic acid capsules are the major virulence factors in Escherichia coliK1, K92, and groups B and C meningococci. The sialic acid monomers (2-keto-3-deoxy-5-acetamido-7,8,9-D-glycero-D-galacto-nonulosonic acids) comprising these homopolymeric polysaccharide chains can be selectively modified with 1,2-diamino-4,5-methylenedioxy-benzene to produce highly fluorescent quinoxalinone derivatives distinguished by their elution times during reverse phase chromatography. Here, we describe methods to release the constituent capsular polysialic acid monomers, and to detect and quantify them by sensitive fluorometry. There are relatively few 2-keto acids in bacteria, making it possible to rapidly analyze samples even without prior purification of capsular polysaccharides.
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Registered trials
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