ArticleIn vitro cellular & developmental biology. Animal2013
Establishment and characterization of a tamoxifen-mediated reversible immortalized mouse dental papilla cell line.
Article in In vitro cellular & developmental biology. Animal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 39 citations in OpenAlex.
- Chromatin Remodeller BRD9 Orchestrates Odontoblastic Differentiation via Coordinating RUNX2-KLF4.Cell proliferation · 2026Article
- CREB3L1 deficiency impairs odontoblastic differentiation and molar dentin deposition partially through the TMEM30B.International journal of oral science · 2024Article
- Intercellular mitochondrial transfer alleviates pyroptosis in dental pulp damage.Cell proliferation · 2023Article
- Immortalized cell lines derived from dental/odontogenic tissue.Cell and tissue research · 2023Review
- Immortalization Reversibility in the Context of Cell Therapy Biosafety.International journal of molecular sciences · 2023Review
- Role of the Demethylase AlkB Homolog H5 in the Promotion of Dentinogenesis.Frontiers in physiology · 2022Article
- Mitochondrial DNA leakage exacerbates odontoblast inflammation through gasdermin D-mediated pyroptosis.Cell death discovery · 2021Article
- Article
- Mitochondrial DNA leakage induces odontoblast inflammation via the cGAS-STING pathway.Cell communication and signaling : CCS · 2021Article
- Klf4 Promotes Dentinogenesis and Odontoblastic Differentiation via Modulation of TGF-β Signaling Pathway and Interaction With Histone Acetylation.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2019Article
- The Dentin Sialoprotein (DSP) Domain Regulates Dental Mesenchymal Cell Differentiation through a Novel Surface Receptor.Scientific reports · 2016Article
- Autophagy regulates odontoblast differentiation by suppressing NF-κB activation in an inflammatory environment.Cell death & disease · 2016Article
- Immortalized mouse dental papilla mesenchymal cells preserve odontoblastic phenotype and respond to bone morphogenetic protein 2.In vitro cellular & developmental biology. Animal · 2013Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Odontoblasts are a type of non-proliferating and terminally differentiated cells that play an important role in the pulpo-dentinal complex. Mouse dental papilla cells (mDPCs), which can differentiate into odontoblast-like cells in vitro, have a limited life span. We combined the traditional strategy of "Cre/LoxP-based reversible immortalization" with a tamoxifen-regulated Cre recombination system to generate a tamoxifen-mediated reversibly immortalized mouse dental papilla cell line, mDPCET. mDPCs were sequentially transduced with a floxed SV40 T antigen-TK (SV40Tag-TK) and an (ERT2)Cre(ERT2)-expressing plasmid. Clonal-isolated SV40Tag- and Cre-positive cells showed modified growth characteristics and a significantly extended life span. When mDPCET cells were treated with 4-hydroxytamoxifen, (ERT2)Cre(ERT2) translocated from the cytoplasm to the nucleus and caused the excision of SV40Tag-TK, which led to the reversion of mDPCETs. After the immortalization was reversed, the cells underwent replicative senescence and transitioned into a more differentiated state. Tamoxifen-mediated reversible immortalization, therefore, allows for the expansion of primary mDPCs, leads to the production of odontoblast-like cells that retain most odontoblast-specific properties, and can represent a safe and ready-to-use method due to its simple manipulation.
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