ArticleBioconjugate chemistry2013
Environment-sensing merocyanine dyes for live cell imaging applications.
Article in Bioconjugate chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 68 citations in OpenAlex.
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- Live-cell biosensors based on the fluorescence lifetime of environment-sensing dyes.Cell reports methods · 2024Article
- Ultrafast photoinduced dynamics of a donor-([Formula: see text])bridge-acceptor based merocyanine dye.Scientific reports · 2022Article
- Quantitative interrogation of protein co-aggregation using multi-color fluorogenic protein aggregation sensors.Chemical science · 2021Article
- Molecular detection of Gram-positive bacteria in the human lung through an optical fiber-based endoscope.European journal of nuclear medicine and molecular imaging · 2021Article
- An Evolutionary Strategy for Identification of Higher Order, Green Fluorescent Host-Guest Pairs Compatible with Living Systems.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Membrane-Permeant, Environment-Sensitive Dyes Generate Biosensors within Living Cells.Journal of the American Chemical Society · 2019Article
- Array-based "Chemical Nose" Sensing in Diagnostics and Drug Discovery.Angewandte Chemie (International ed. in English) · 2019Review
- Epigallocatechin gallate has pleiotropic effects on transmembrane signaling by altering the embedding of transmembrane domains.The Journal of biological chemistry · 2017Article
- The Cation-π Interaction Enables a Halo-Tag Fluorogenic Probe for Fast No-Wash Live Cell Imaging and Gel-Free Protein Quantification.Biochemistry · 2017Article
- Design Principles for SuCESsFul Biosensors: Specific Fluorophore/Analyte Binding and Minimization of Fluorophore/Scaffold Interactions.Journal of molecular biology · 2016Article
- Labelling Bacterial Nanocages with Photo-switchable Fluorophores.Chemphyschem : a European journal of chemical physics and physical chemistry · 2016Article
- Using membrane composition to fine-tune the pJournal of materials chemistry. C · 2016Article
- Far-red/near-infrared fluorescence light-up probes for specific in vitro and in vivo imaging of a tumour-related protein.Scientific reports · 2016Article
- Ratiometric Imaging Using a Single Dye Enables Simultaneous Visualization of Rac1 and Cdc42 Activation.Journal of the American Chemical Society · 2016Article
- A novel fluorescent sensor protein for detecting changes in airway surface liquid glucose concentration.The Biochemical journal · 2014Article
- Knowledge-based design of a biosensor to quantify localized ERK activation in living cells.Chemistry & biology · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Fluorescent biosensors based on environmentally sensitive dyes enable visualization and quantification of endogenous protein activation within living cells. Merocyanine dyes are especially useful for live cell imaging applications, as they are extraordinarily bright, have long wavelengths of excitation and emission, and can exhibit readily detectable fluorescence changes in response to environment. We sought to systematically examine the effects of structural features on key photophysical properties, including dye brightness, environmental responsiveness, and photostability, through the synthesis of a library of 25 merocyanine dyes, derived from combinatorial reaction of 5 donor and 5 acceptor heterocycles. Four of these dyes showed optimal properties for specific imaging applications and were subsequently prepared with reactive side chains and enhanced aqueous solubility using a one-pot synthetic method. The new dyes were then applied within a biosensor design for Cdc42 activation, where dye mero60 showed a remarkable 1470% increase in fluorescence intensity on binding activated Cdc42 in vitro. The dye-based biosensors were used to report activation of endogenous Cdc42 in living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.