ArticleThe Journal of biological chemistry2013
The phosphatidic acid binding site of the Arabidopsis trigalactosyldiacylglycerol 4 (TGD4) protein required for lipid import into chloroplasts.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 58 citations in OpenAlex.
- Biochemical characterisation supports a central role for Oep80 from Arabidopsis thaliana in chloroplastic β-barrel protein assembly.Communications biology · 2025Article
- Review
- Drought and heat stress mediated activation of lipid signaling in plants: a critical review.Frontiers in plant science · 2023Review
- Fatty acid desaturases (FADs) modulate multiple lipid metabolism pathways to improve plant resistance.Molecular biology reports · 2022Review
- Trigalactosyldiacylglycerol 3 protein orthologs are required for basal disease resistance inPlant biotechnology (Tokyo, Japan) · 2021Article
- Review
- New Era of Diacylglycerol Kinase, Phosphatidic Acid and Phosphatidic Acid-Binding Protein.International journal of molecular sciences · 2020Review
- Non-intrinsic ATP-binding cassette proteins ABCI19, ABCI20 and ABCI21 modulate cytokinin response at the endoplasmic reticulum in Arabidopsis thaliana.Plant cell reports · 2020Article
- Identification and evaluation of reference genes for reliable normalization of real-time quantitative PCR data in acerola fruit, leaf, and flower.Molecular biology reports · 2020Article
- The Puzzle of Metabolite Exchange and Identification of Putative Octotrico Peptide Repeat Expression Regulators in the Nascent Photosynthetic Organelles ofFrontiers in microbiology · 2020Article
- Metabolically Distinct Pools of Phosphatidylcholine Are Involved in Trafficking of Fatty Acids out of and into the Chloroplast for Membrane Production.The Plant cell · 2019Article
- A predicted plastid rhomboid protease affects phosphatidic acid metabolism in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2019Article
- Intermembrane transport: Glycerophospholipid homeostasis of the Gram-negative cell envelope.Proceedings of the National Academy of Sciences of the United States of America · 2019Review
- Cellular Organization and Regulation of Plant Glycerolipid Metabolism.Plant & cell physiology · 2019Review
- Lipid transport required to make lipids of photosynthetic membranes.Photosynthesis research · 2018Review
- Lipid somersaults: Uncovering the mechanisms of protein-mediated lipid flipping.Progress in lipid research · 2016Review
- FAX1, a novel membrane protein mediating plastid fatty acid export.PLoS biology · 2015Article
- Phosphatidic acid, a versatile water-stress signal in roots.Frontiers in plant science · 2013Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chloroplast membrane lipid synthesis relies on the import of glycerolipids from the ER. The TGD (TriGalactosylDiacylglycerol) proteins are required for this lipid transfer process. The TGD1, -2, and -3 proteins form a putative ABC (ATP-binding cassette) transporter transporting ER-derived lipids through the inner envelope membrane of the chloroplast, while TGD4 binds phosphatidic acid (PtdOH) and resides in the outer chloroplast envelope. We identified two sequences in TGD4, amino acids 1-80 and 110-145, which are necessary and sufficient for PtdOH binding. Deletion of both sequences abolished PtdOH binding activity. We also found that TGD4 from 18:3 plants bound specifically and with increased affinity PtdOH. TGD4 did not interact with other proteins and formed a homodimer both in vitro and in vivo. Our results suggest that TGD4 is an integral dimeric β-barrel lipid transfer protein that binds PtdOH with its N terminus and contains dimerization domains at its C terminus.
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