ArticleThe Journal of biological chemistry2013
Proteomic analysis reveals that the Rab GTPase RabE1c is involved in the degradation of the peroxisomal protein receptor PEX7 (peroxin 7).
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- Antibody array-based proteome approach reveals proteins involved in grape seed development.Plant physiology · 2024Article
- Molecular Machinery of Lipid Droplet Degradation and Turnover in Plants.International journal of molecular sciences · 2023Review
- Rab GTPases, tethers, and SNAREs work together to regulateFrontiers in plant science · 2023Review
- Mapping-by-Sequencing Reveals Genomic Regions Associated with Seed Quality Parameters inGenes · 2022Article
- QTL mapping and candidate gene prediction for fiber yield and quality traits in a high-generation cotton chromosome substitution line with Gossypium barbadense segments.Molecular genetics and genomics : MGG · 2022Article
- Article
- Possible Roles of Membrane Trafficking Components for Lipid Droplet Dynamics in Higher Plants and Green Algae.Frontiers in plant science · 2019Review
- Peroxisome Function, Biogenesis, and Dynamics in Plants.Plant physiology · 2018Review
- Plant peroxisomes: recent discoveries in functional complexity, organelle homeostasis, and morphological dynamics.Current opinion in plant biology · 2016Review
- Genetic Interactions between PEROXIN12 and Other Peroxisome-Associated Ubiquitination Components.Plant physiology · 2016Article
- Sucrose Production Mediated by Lipid Metabolism Suppresses the Physical Interaction of Peroxisomes and Oil Bodies during Germination of Arabidopsis thaliana.The Journal of biological chemistry · 2016Article
- Covalent Label Transfer between Peroxisomal Importomer Components Reveals Export-driven Import Interactions.The Journal of biological chemistry · 2016Article
- The unique degradation pathway of the PTS2 receptor, Pex7, is dependent on the PTS receptor/coreceptor, Pex5 and Pex20.Molecular biology of the cell · 2014Article
- RabGAP22 is required for defense to the vascular pathogen Verticillium longisporum and contributes to stomata immunity.PloS one · 2014Article
- Highly oxidized peroxisomes are selectively degraded via autophagy in Arabidopsis.The Plant cell · 2013Article
- Proteome analysis of peroxisomes from etiolated Arabidopsis seedlings identifies a peroxisomal protease involved in β-oxidation and development.Plant physiology · 2013Article
- Novel proteins interacting with peroxisomal protein receptor PEX7 in Arabidopsis thaliana.Plant signaling & behavior · 2013Article
- Import of proteins into the peroxisomal matrix.Frontiers in physiology · 2013Review
- Peroxisome degradation in mammals: mechanisms of action, recent advances, and perspectives.Frontiers in physiology · 2013Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The biogenesis of peroxisomes is mediated by peroxins (PEXs). PEX7 is a cytosolic receptor that imports peroxisomal targeting signal type 2 (PTS2)-containing proteins. Although PEX7 is important for protein transport, the mechanisms that mediate its function are unknown. In this study, we performed proteomic analysis to identify PEX7-binding proteins using transgenic Arabidopsis expressing green fluorescent protein (GFP)-tagged PEX7. Our analysis identified RabE1c, a small GTPase, as a PEX7 binding partner. In vivo analysis revealed that GTP-bound RabE1c binds to PEX7 and that a subset of RabE1c localizes to peroxisomes and interacts with PEX7 on the peroxisome membrane. Unlike endogenous PEX7, which is predominantly localized to the cytosol, GFP-PEX7 accumulates abnormally on the peroxisomal membrane and induces degradation of endogenous PEX7, concomitant with a reduction in import of PTS2-containing proteins and decreased peroxisomal β-oxidation activity. Thus, GFP-PEX7 on the peroxisomal membrane exerts a dominant negative effect. Mutation of RabE1c restored endogenous PEX7 protein expression and import of PTS2-containing proteins as well as peroxisomal β-oxidation activity. Treatment with proteasome inhibitors also restored endogenous PEX7 protein levels in GFP-PEX7-expressing seedlings. Based on these findings, we conclude that RabE1c binds PEX7 and facilitates PEX7 degradation in the presence of immobile GFP-PEX7 accumulated at the membrane.
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