ArticleThe Journal of biological chemistry2013
Nitric-oxide synthase-2 linkage to focal adhesion kinase in neutrophils influences enzyme activity and β2 integrin function.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- Redox Regulation of Endogenous Gasotransmitters in Vascular Health and Disease.International journal of molecular sciences · 2025Review
- COL12A1 Promotes Osteosarcoma Progression via the FAK/PI3K/AKT/mTOR Pathway.Current molecular medicine · 2025Article
- Effects of Pressure, Hypoxia, and Hyperoxia on Neutrophil Granulocytes.Biomolecules · 2024Article
- Dissolved gases from pressure changes in the lungs elicit an immune response in human peripheral blood.Bioengineering & translational medicine · 2024Article
- Inhaled nitric oxide suppresses neuroinflammation in experimental ischemic stroke.Journal of neuroinflammation · 2023Article
- Dissolved gases from pressure changes in the lungs elicit an immune response in human peripheral blood.bioRxiv : the preprint server for biology · 2023Article
- Nitric Oxide and Electrophilic Cyclopentenone Prostaglandins in Redox signaling, Regulation of Cytoskeleton Dynamics and Intercellular Communication.Frontiers in cell and developmental biology · 2021Review
- Pathogenicity & virulence ofVirulence · 2020Review
- Toddalolactone Protects Lipopolysaccharide-Induced Sepsis and Attenuates Lipopolysaccharide-Induced Inflammatory Response by Modulating HMGB1-NF-κB Translocation.Frontiers in pharmacology · 2020Article
- Role of NO and S-nitrosylation in the Expression of Endothelial Adhesion Proteins That Regulate Leukocyte and Tumor Cell Adhesion.Frontiers in physiology · 2020Review
- Microparticle and interleukin-1β production with human simulated compressed air diving.Scientific reports · 2019Article
- Identification of new binding proteins of focal adhesion kinase using immunoprecipitation and mass spectrometry.Scientific reports · 2019Article
- Resveratrol is a promising agent for colorectal cancer prevention and treatment: focus on molecular mechanisms.Cancer cell international · 2019Review
- Neutrophil microparticle production and inflammasome activation by hyperglycemia due to cytoskeletal instability.The Journal of biological chemistry · 2017Article
- Factors Associated with Nitric Oxide-mediated β2 Integrin Inhibition of Neutrophils.The Journal of biological chemistry · 2015Article
- Article
- Hyperbaric oxygen, vasculogenic stem cells, and wound healing.Antioxidants & redox signaling · 2014Review
- Neutrophils generate microparticles during exposure to inert gases due to cytoskeletal oxidative stress.The Journal of biological chemistry · 2014Article
- Polydatin, a natural precursor of resveratrol, induces cell cycle arrest and differentiation of human colorectal Caco-2 cell.Journal of translational medicine · 2013Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This investigation was to elucidate the basis for augmentation of nitric-oxide synthesis in neutrophils exposed to hyperbaric oxygen. Hyperoxia increases synthesis of reactive species leading to S-nitrosylation of β-actin, which causes temporary inhibition of β(2) integrin adherence. Impaired β(2) integrin function and actin S-nitrosylation do not occur in neutrophils from mice lacking type-2 nitric-oxide synthase (iNOS) or when incubated with 1400W, an iNOS inhibitor. Similarly, effects of hyperoxia were abrogated in cells depleted of focal adhesion kinase (FAK) by treatment with small inhibitory RNA and those exposed to a specific FAK inhibitor concurrent with hyperoxia. Nitric oxide production doubles within 10 min exposure to hyperoxia but declines to approximately half-maximum production over an additional 10 min. Elevated nitric oxide production did not occur after FAK depletion or inhibition, or when filamentous actin formation was inhibited by cytochalasin D. Intracellular content of iNOS triples over the course of a 45-min exposure to hyperoxia and iNOS dimers increase in a commensurate fashion. Confocal microscopy and immunoprecipitation demonstrated that co-localization/linkage of FAK, iNOS, and filamentous actin increased within 15 min exposure to hyperoxia but then decreased below the control level. Using isolated enzymes in ex vivo preparations an association between iNOS and filamentous actin mediated by FAK could be demonstrated and complex formation was impeded when actin was S-nitrosylated. We conclude that iNOS activity is increased by an FAK-mediated association with actin filaments but peak nitric oxide production is transient due to actin S-nitrosylation during exposure to hyperoxia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.