ArticleProceedings of the National Academy of Sciences of the United States of America2013
SLC4 family transporters in a marine diatom directly pump bicarbonate from seawater.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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65 citing papers in PubMed, 160 citations in OpenAlex.
- Role of extracellular carbonic anhydrase in the polar diatom Fragilariopsis cylindrus.The New phytologist · 2026Article
- Architectural and biochemical advances in understanding microbial COThe Plant journal : for cell and molecular biology · 2026Review
- Comprehensive identification and subcellular localization prediction of carbonic anhydrases in the marine haptophyte Tisochrysis lutea based on a refined genome annotation.Archives of microbiology · 2026Article
- Dynamic changes in phycosphere carbonate chemistry reveal rapid modulation of carbon uptake in single diatom cells.Science advances · 2026Article
- Interactions with bacteria shape diatom adaptation to carbon concentration changes.Nature communications · 2025Article
- Inorganic Carbon Acquisition and Photosynthetic Metabolism in Marine Photoautotrophs: A Summary.Plants (Basel, Switzerland) · 2025Review
- Stage-dependent changes in culture medium osmolality promote porcine oocyte maturationFrontiers in cell and developmental biology · 2025Article
- Molecular complexity of the differential growth of freshwater diatoms along pH gradients.ISME communications · 2025Article
- Article
- Mixotrophic growth of a ubiquitous marine diatom.Science advances · 2024Article
- Modification of Living Diatom,ACS applied bio materials · 2024Article
- Low-CO2-inducible bestrophins outside the pyrenoid sustain high photosynthetic efficacy in diatoms.Plant physiology · 2024Article
- Transportation for fuel: Thylakoid membrane bestrophin channels facilitate HCO3- transport to the pyrenoid in diatoms.Plant physiology · 2024Article
- Coordinated wound responses in a regenerative animal-algal holobiont.Nature communications · 2024Article
- Cooperation of an external carbonic anhydrase and HCO3- transporter supports underwater photosynthesis in submerged leaves of the amphibious plant Hygrophila difformis.Annals of botany · 2024Article
- Extra OPhotosynthesis research · 2024Article
- Short-term acidification promotes diverse iron acquisition and conservation mechanisms in upwelling-associated phytoplankton.Nature communications · 2023Article
- The V-type ATPase enhances photosynthesis in marine phytoplankton and further links phagocytosis to symbiogenesis.Current biology : CB · 2023Article
- Temperature sensitivity of carbon concentrating mechanisms in the diatom Phaeodactylum tricornutum.Photosynthesis research · 2023Article
- Localization and characterization θ carbonic anhydrases in Thalassiosira pseudonana.Photosynthesis research · 2023Article
5 more citing papers are in PubMed but not listed here.
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Photosynthesis in marine diatoms is a vital fraction of global primary production empowered by CO(2)-concentrating mechanisms. Acquisition of HCO(3)(-) from seawater is a critical primary step of the CO(2)-concentrating mechanism, allowing marine photoautotrophic eukaryotes to overcome CO(2) limitation in alkaline high-salinity water. However, little is known about molecular mechanisms governing this process. Here, we show the importance of a plasma membrane-type HCO(3)(-) transporter for CO(2) acquisition in a marine diatom. Ten putative solute carrier (SLC) family HCO(3)(-) transporter genes were found in the genome of the marine pennate diatom Phaeodactylum tricornutum. Homologs also exist in marine centric species, Thalassiosira pseudonana, suggesting a general occurrence of SLC transporters in marine diatoms. Seven genes were found to encode putative mammalian-type SLC4 family transporters in P. tricornutum, and three of seven genes were specifically transcribed under low CO(2) conditions. One of these gene products, PtSLC4-2, was localized at the plasmalemma and significantly stimulated both dissolved inorganic carbon (DIC) uptake and photosynthesis in P. tricornutum. DIC uptake by PtSLC4-2 was efficiently inhibited by an anion-exchanger inhibitor, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid, in a concentration-dependent manner and highly dependent on Na(+) ions at concentrations over 100 mM. These results show that DIC influx into marine diatoms is directly driven at the plasmalemma by a specific HCO(3)(-) transporter with a significant halophilic nature.
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