ArticleProceedings of the National Academy of Sciences of the United States of America2013
Nascent-Seq analysis of Drosophila cycling gene expression.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
62 citing papers in PubMed, 93 citations in OpenAlex.
- Analysis of host and viral nascent and steady-state RNA levels in a human neuronal model of herpes simplex virus 1 infection.PLoS pathogens · 2026Article
- Disordered but rhythmic-the role of intrinsic protein disorder in eukaryotic circadian timing.FEBS letters · 2026Review
- Remote and partial clocks expand the circadian neuronal network, driving widespread molecular rhythmicity inbioRxiv : the preprint server for biology · 2025Article
- Light-regulated microRNAs shape dynamic gene expression in the zebrafish circadian clock.PLoS genetics · 2025Article
- Uncovering the circadian transcriptome ofProceedings. Biological sciences · 2024Article
- Regulation of protein O-GlcNAcylation by circadian, metabolic, and cellular signals.The Journal of biological chemistry · 2024Review
- Nonlinear expression patterns and multiple shifts in gene network interactions underlie robust phenotypic change in Drosophila melanogaster selected for night sleep duration.PLoS computational biology · 2023Article
- Organismal landscape of clock cells and circadian gene expression inbioRxiv : the preprint server for biology · 2023Article
- Butt-seq: a new method for facile profiling of transcription.Genes & development · 2023Article
- Nutrient-sensitive protein O-GlcNAcylation shapes daily biological rhythms.Open biology · 2022Review
- Roles of peripheral clocks: lessons from the fly.FEBS letters · 2022Review
- Discovery and Visualization of Age-Dependent Patterns in the Diurnal Transcriptome of Drosophila.Journal of circadian rhythms · 2022Article
- Identification of Genes Contributing to a Long Circadian Period inJournal of biological rhythms · 2021Article
- The Making and Breaking of RNAs: Dynamics of Rhythmic RNA Expression in Mammals.Journal of biological rhythms · 2020Review
- Screening and functional prediction of differentially expressed circRNAs in proliferative human aortic smooth muscle cells.Journal of cellular and molecular medicine · 2020Article
- Splicing the Clock to Maintain and Entrain Circadian Rhythms.Journal of biological rhythms · 2019Review
- Extensive transcriptional responses are co-ordinated by microRNAs as revealed by Exon-Intron Split Analysis (EISA).Nucleic acids research · 2019Article
- Genetics of Circadian Rhythms.Neurologic clinics · 2019Review
- Genome-wide discovery of the daily transcriptome, DNA regulatory elements and transcription factor occupancy in the monarch butterfly brain.PLoS genetics · 2019Article
- Daily Variations in the Expression of Genes Related to Insecticide Resistance in the Chagas Disease VectorThe American journal of tropical medicine and hygiene · 2019Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Rhythmic mRNA expression is a hallmark of circadian biology and has been described in numerous experimental systems including mammals. A small number of core clock gene mRNAs and a much larger number of output mRNAs are under circadian control. The rhythmic expression of core clock genes is regulated at the transcriptional level, and this regulation is important for the timekeeping mechanism. However, the relative contribution of transcriptional and post transcriptional regulation to global circadian mRNA oscillations is unknown. To address this issue in Drosophila, we isolated nascent RNA from adult fly heads collected at different time points and subjected it to high-throughput sequencing. mRNA was isolated and sequence din parallel. Some genes had cycling nascent RNAs with no cycling mRNA, caused,most likely, by light-mediated read-through transcription. Most genes with cycling mRNAs had significant nascent RNA cycling amplitudes, indicating a prominent role for circadian transcriptional regulation. However, a considerable fraction had higher mRNA amplitudes than nascent RNA amplitudes. The same comparison for core clock gene mRNAs gives rise to a qualitatively similar conclusion. The data therefore indicate a significant quantitative contribution of post transcriptional regulation to mRNA cycling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.