ArticleProceedings of the National Academy of Sciences of the United States of America2013
Extracellular transmission of a DNA mycovirus and its use as a natural fungicide.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 153 papers.
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Who cites it
153 citing papers in PubMed, 320 citations in OpenAlex.
- Molecular Characterization of a Novel Mycovirus Isolated from Binucleate Rhizoctonia AG‑Bb strain 8-Q-36.Current microbiology · 2026Article
- A Novel Mycovirus Reprograms the Pathogenic State of Aspergillus flavus in Keratitis.Investigative ophthalmology & visual science · 2026Article
- Mycoviruses against plant pathogenic fungi: current understanding and future avenues of their potential implication.Archives of microbiology · 2026Review
- Expanding the Antimicrobial Toolbox with Therapeutic Viruses: Mechanisms, Pharmaceutical Formulation, and Translational Outlook.Pharmaceuticals (Basel, Switzerland) · 2026Review
- Mycoviruses: Environmental Variables, Vector-Mediated Transmission and Use as a Biocontrol.Viruses · 2026Review
- Sclerotinia sclerotiorum growth and aggressiveness are regulated by a mycoviral REP protein.PLoS pathogens · 2025Article
- Viral driving force on virulence differentiation of a rice phytopathogenic fungus and the implication for biocontrol.Virulence · 2025Article
- The Role of Phyllosphere Microbes and Viruses in Biocontrol of Pathogenic Fungi.Microbial biotechnology · 2025Review
- Molecular characterization of four novel mycoviruses in entomopathogenic and nematophagous fungi.Virology journal · 2025Article
- Cloning and Characterization of the Mycovirus MpChrV2 fromJournal of fungi (Basel, Switzerland) · 2025Article
- Molecular characterization of a novel chrysovirus isolated from the phytopathogenic fungus Fusarium commune.Archives of virology · 2025Article
- High-Throughput Sequencing Reveals the Mycoviral Diversity of the Pathogenic Grape FungusViruses · 2025Article
- Identification and characterization of a novel negative-sense RNA virus infecting Fusarium commune, a fungus causing tobacco root rot.Archives of virology · 2025Article
- Effects of Thermal and Antibiotic Treatments on the Viral Accumulation of FcMV1 inJournal of fungi (Basel, Switzerland) · 2025Article
- Molecular characterization of a novel non-segmented double-stranded RNA mycovirus isolated from the phytopathogenic fungus Lasiodiplodia pseudotheobromae.Archives of virology · 2025Article
- Molecular and Biological Characterization of an Isolate of Fusarium graminearum dsRNA mycovirus 4 (FgV4) from a New HostMicroorganisms · 2025Article
- Molecular characterization of a novel deltaflexivirus from the plant-pathogenic fungus Neopestalotiopsis nebuloides strain N-7.Archives of virology · 2025Article
- Discovery of novel mycoviruses from fungi associated with mango leaf spots.Frontiers in microbiology · 2025Article
- Diverse, novel mycoviruses coinfecting the phytopathogenic fungusFrontiers in cellular and infection microbiology · 2025Article
- Identification of a novel hypovirulence-inducing ourmia-like mycovirus fromFrontiers in microbiology · 2025Article
93 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mycoviruses are thought not to be infectious as free particles and to lack an extracellular phase in their life cycles, limiting the broad use of hypovirulence-associated mycoviruses in controlling fungal disease. Here, we demonstrate that purified particles of a DNA mycovirus, Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1), are infectious when applied extracellularly to its host Sclerotinia sclerotiorum. Virus particles isolated from an infected host can infect the hyphae of virus-free S. sclerotiorum directly when applied to hyphae grown on potato dextrose agar or sprayed on leaves of Arabidopsis thaliana and Brassica napus, regardless of vegetative compatibility affiliation. When applied to leaves, the virus can suppress the development of lesions. SsHADV-1 can also reduce disease severity and enhance rapeseed yield significantly under field conditions. SsHADV-1 has a narrow host range; it can infect Sclerotinia minor and Sclerotinia nivalis, sister species of S. sclerotiorum, and cause debilitation of these two fungi, but cannot infect or transfect other tested fungi, such as Botrytis cinerea, which shares the same family with S. sclerotiorum. Virus particles are likely to be very stable on the leaves of A. thaliana plants because viral DNA could be detected at 15 d postinoculation on unwounded leaves and at 10 d postinoculation on wounded leaves, respectively; however, this virus could not infect and move in plant cells. Our findings may prompt a reconsideration of the generalization that mycoviruses lack an extracellular phase in their life cycles and stimulate the search for other DNA mycoviruses with potential use as natural fungicides.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.